Kapasitas Antioksidan dan Inhibisi Lipase Pankreas Kombinasi Daun Kumis Kucing (Orthosiphon aristatus) dan Daun Jambu Biji (Psidium guajava) secara In Vitro

Abstract

Obesitas merupakan kondisi kesehatan yang ditandai dengan akumulasi lemak berlebih dalam tubuh dan prevalensinya terus meningkat secara global. Kumis kucing (Orthosiphon aristatus) dan jambu biji (Psidium guajava) merupakan tanaman yang berpotensi sebagai antiobesitas melalui inhibisi pada enzim lipase pankreas. Penelitian ini bertujuan menentukan kadar fenolik, kadar flavonoid, kapasitas antioksidan, dan aktivitas inhibisi lipase pankreas, serta interaksi yang terjadi pada kombinasi ekstrak daun kumis kucing dengan ekstrak daun jambu biji. Ekstraksi dilakukan dengan metode sonikasi dan hasil ekstraksi dikombinasikan dalam tujuh formula berbeda. Setiap formulasi diuji untuk kadar total fenolik, flavonoid, kapasitas antioksidan menggunakan metode DPPH dan FRAP, serta aktivitas inhibisi terhadap enzim lipase pankreas. Hasil penelitian menunjukkan bahwa formulasi yang mengandung ekstrak jambu biji secara tunggal memiliki kadar total fenolik dan flavonoid tertinggi, serta aktivitas antioksidan dan inhibisi lipase pankreas paling kuat. Namun, nilai inhibisi lipase pankreas dari ketujuh formulasi relatif serupa, yaitu berkisar antara 62% hingga 69%.

Obesity is a health condition characterized by the accumulation of excess fat in the body and its prevalence continues to increase globally. Cat's whiskers (Orthosiphon aristatus) and guava (Psidium guajava) are plants that have the potential as antiobesity through inhibition of pancreatic lipase enzymes. This study aims to determine the phenolic content, flavonoid content, antioxidant capacity, and pancreatic lipase inhibition activity, as well as the interactions that occur in the combination of cat's whiskers leaf extract with guava leaf extract. Extraction was carried out using the sonication method and the extraction results were combined in seven different formulas. Each formulation was tested for total phenolic content, flavonoids, antioxidant capacity using the DPPH and FRAP methods, and inhibitory activity against pancreatic lipase enzymes. The results showed that the formulation containing guava extract alone had the highest total phenolic and flavonoid content, as well as the strongest antioxidant and pancreatic lipase inhibition activities. However, the pancreatic lipase inhibition values of the seven formulations were relatively similar, ranging from 62% to 69%.