Validasi Metode Penetapan Kadar Niasinamida dalam Kosmetika Semi Padat dengan KCKT-UV di Laboratorium LPPOM MUI

Abstract

Niasinamida sering digunakan dalam kosmetika karena manfaatnya bagi kulit. Penetapan kadarnya secara akurat dalam kosmetika semi padat penting untuk memastikan kualitas produk. Penetapan kadar niasinamida dalam kosmetika semi padat belum tersedia sebagai metode rutin di laboratorium LPPOM MUI Bogor. Validasi metode KCKT-UV dilakukan untuk analisis niasinamida agar metode tersebut layak digunakan dalam pengujian rutin. Hasil menunjukkan metode ini spesifik untuk niasinamida dengan BDI 0,020 mg/L, BD 4,980 mg/kg, dan BK 19,920 mg/kg. Kurva kalibrasi linearitas dan rentang kerja memiliki koefisien korelasi (r) 0,9998. Presisi keterulangan menghasilkan %SBR = 2/3 CV Horwitz (0,46 = 3,85). Presisi antara tidak terdapat perbedaan signifikan antar analis. Akurasi pada konsentrasi 80%, 100%, dan 120% menunjukkan %perolehan kembali antara 95–105%. Pengujian ketegaran menunjukkan bahwa bobot penimbangan dan waktu pengukuran tidak memengaruhi hasil. Metode ini valid untuk penetapan kadar niasinamida dalam kosmetika semi padat.

Niacinamide was frequently utilized in cosmetic products due to its beneficial effects on the skin. Accurate quantification of niacinamide in semi-solid cosmetics was essential to ensure product quality. A routine method for determining niacinamide content in semi-solid cosmetics was not yet established at the LPPOM MUI Laboratory in Bogor. The HPLC-UV method was validated for niacinamide analysis to ensure its suitability for routine testing. The results indicated that the method was specific for niacinamide, with an IDL of 0.020 mg/L, a LOD of 4.980 mg/kg, and a LOQ of 19.920 mg/kg. The calibration curve for linearity and working range yielded a correlation coefficient (r) of 0.9998. Repeatability precision produced a %RSD = 2/3 CV Horwitz (0.46 = 3.85). Intermediate precision revealed no significant differences between analysts. Accuracy at concentrations of 80%, 100%, and 120% demonstrated recoveries ranging from 95% to 105%. Robustness testing indicated that weighing mass and measurement time did not affect the results. This method was valid for the determination of niacinamide content in semi-solid cosmetics.