Uji Antioksidan dan Inhibisi Lipase Pankreas Kombinasi Ekstrak Buah Asam Gelugur dan Daun Asam Jawa Secara in Vitro

Abstract

Obesitas telah menjadi masalah kesehatan global dan obat alternatif Orlistat memiliki efek samping yang tidak diinginkan. Penelitian bertujuan menguji total fenolik, total flavonoid, kapasitas antioksidan, dan inhibisi lipase pankreas kombinasi ekstrak kasar etanol buah asam gelugur (Garcinia atroviridis) dan daun asam jawa (Tamarindus indica) secara in vitro. Metode melibatkan ekstraksi, formulasi kombinasi ekstrak, dan pengujian in vitro total fenolik, total flavonoid, kapasitas antioksidan (DPPH, FRAP), serta inhibisi lipase pankreas. Hasil kadar fenolik total memberikan efek antagonis dengan penurunan signifikan pada kombinasi 1:1 sebesar 409,146 ± 27,156 mg GAE/g. Kadar flavonoid total memberikan efek sinergis dengan kenaikan pada kombinasi 1:1 sebesar 13,227 ± 0,498 mg QE/g. Kapasitas antioksidan FRAP berefek aditif dan DPPH berefek antagonis. Inhibisi lipase pankreas tak menunjukkan perbedaan signifikan dengan persentase inhibisi rata-rata di rentang 70-77%.

Obesity has become a global health problem and alternative drug Orlistat has undesirable side effects. The study tested the total phenolic, total flavonoid, antioxidant capacity, and pancreatic lipase inhibition of a combination of crude ethanol extracts of Garcinia atroviridis fruit and Tamarindus indica leaves in vitro. The methodology involved extraction, formulation of extract combinations, in vitro testing for total phenolics, total flavonoids, antioxidant capacity (DPPH, FRAP), and pancreatic lipase inhibition. The results of total phenolic content gave an antagonistic effect with a significant decrease in the 1:1 combination of 409.146 ± 27.156 mg GAE/g. Total flavonoid levels gave a synergistic effect with an increase in the 1:1 combination of 13,227 ± 0.498 mg QE/g. Antioxidant capacity of FRAP had additive effect and DPPH had antagonistic effect. Pancreatic lipase inhibition showed no significant difference with inhibition percentage ranging from 70-77%.