Pola Resistansi Klebsiella Sp. Yang Diisolasi Dari Feses Kuda (Equus caballus) Di Unit Rehabilitasi Reproduksi, SKHB IPB

Abstract

Kuda merupakan salah satu hewan yang banyak digunakan dalam aktivitas manusia. Salah satu bakteri yang telah diidentifikasi dari feses kuda dan dilaporkan telah mengalami resistansi terhadap banyak antibiotik adalah Klebsiella sp. Penelitian ini bertujuan mengidentifikasi bakteri Klebsiella sp. yang diisolasi dari feses kuda (Equus caballus) dan menganalisis pola resistansinya terhadap beberapa jenis antibiotik. Sebanyak 17 sampel feses kuda diisolasi dan dilakukan pengamatan karakteristik koloni bakteri, morfologi, dan uji biokimia sehingga teridentifikasi sembilan (52,9%) isolat yang memiliki karakteristik sesuai dengan Klebsiella. Pada sembilan isolat tersebut, dilakukan uji resistansi antibiotik dengan metode difusi cakram (Kirby Bauer test) terhadap lima antibiotik. Hasil pengujian ditemukan isolat yang intermediate terhadap ampisilin sebanyak 11,1% (1/9) dan seftazidim sebanyak 33,3% (3/9). Ditemukan juga isolat yang resistan terhadap ampisilin sebanyak 44,4% (4/9) dan seftazidim sebanyak 11,1% (1/9). Semua isolat (100%) ditemukan masih sensitif terhadap gentamisin, tetrasiklin, dan siprofloksasin. Tidak ditemukan isolat Klebsiella yang dikategorikan multidrug-resistant (MDR).

Horses are one of the animals that are widely used in human activities. One of the bacteria that has been identified from horse feces and reported to have developed resistance to many antibiotics is Klebsiella sp. This study aims to identify Klebsiella sp. bacteria isolated from horse (Equus caballus) feces and analyze their resistance patterns to several types of antibiotics. A total of 17 horse fecal samples were isolated and examined based on bacterial colony characteristics, morphology, and biochemical tests, resulting in the identification of nine (52,9%) isolates with characteristics consistent with Klebsiella. The nine isolates were tested for antibiotic resistance using the disc diffusion method (Kirby Bauer test) against five antibiotics. The test results showed that 11.1% (1/9) of the isolates were intermediate to ampicillin and 33.3% (3/9) were intermediate to ceftazidime. This study also found that 44.4% (4/9) of the isolates were resistant to ampicillin and 11.1% (1/9) were resistant to ceftazidime. All isolates (100%) were found to remain sensitive to gentamicin, tetracycline, and ciprofloxacin. No Klebsiella isolates were identified as multidrug-resistant (MDR).