Please use this identifier to cite or link to this item: http://repository.ipb.ac.id/handle/123456789/163798
Title: Optimasi Pelarut Ekstraksi Andaliman (Zanthoxylum acanthopodium DC.) terhadap Kandungan Polifenol dan Aktivitas Antioksidan dengan Simplex Centroid Design.
Other Titles: Optimization of Andaliman (Zanthoxylum acanthopodium DC.) Extraction Solvents on Polyphenol Content and Antioxidant Activity using Simplex Centroid Design
Authors: Nurcholis, Waras
Diantina, Surya
Septiawati, Indah Zahra
Issue Date: 2025
Publisher: IPB University
Abstract: Andaliman (Z. acanthopodium DC.) adalah tanaman rempah yang berpotensi sebagai antioksidan karena mengandung fenolik dan flavonoid. Penelitian ini bertujuan menentukan jenis pelarut ekstraksi terbaik untuk mengekstrak daun andaliman berdasarkan kadar total fenolik, flavonoid, dan aktivitas antioksidan. Sampel diekstraksi dengan metode sonikasi-maserasi. Pengolahan data menggunakan aplikasi Design Expert 13.0 dan SPSS IBM. Hasil penelitian menunjukkan bahwa model kuadratik merupakan hasil paling optimum untuk memprediksi semua variabel respon. Hasil verifikasi model menunjukkan kombinasi tiga pelarut dengan rasio air : etanol : aseton sebesar 0,360 : 0,015 : 0,625. Verifikasi optimum tersebut menghasilkan nilai aktual respon TPC 35,137 mg GAE/g DW, TFC 4,564 mg QE/g DW, DPPH 1,747 µmol TE/g DW, dan FRAP 35,904 µmol TE/g DW. Simpulan penelitian ini yaitu SCD dapat mengoptimalkan pelarut ekstraksi daun andaliman dengan respon kadar total fenolik, flavonoid, dan aktivitas antioksidan.
Andaliman (Z. acanthopodium DC.) is a spice plant that has the potential as an antioxidant because it contains phenolics and flavonoids. This study aims to determine the best type of extraction solvent to extract andaliman leaves based on the total phenolic, flavonoid, and antioxidant activity. The sample was extracted using the sonication-maceration method. Data processing used the Design Expert 13.0 application and IBM SPSS. The results showed that the quadratic model was the most optimum result for predicting all response variables. The results of model verification showed a combination of three solvents with a water : ethanol : acetone ratio of 0,360 : 0,015 : 0,625. The optimum verification produced actual values of TPC response of 35.137 mg GAE/g DW, TFC 4.564 mg QE/g DW, DPPH 1.747 µmol TE/g DW, and FRAP 35.904 µmol TE/g DW. The conclusion of this research is that the Simplex Centroid Design can optimize the andaliman leaf extraction solvent with a response to the total levels of phenolics, flavonoids and antioxidant activity.
URI: http://repository.ipb.ac.id/handle/123456789/163798
Appears in Collections:UT - Biochemistry

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