Kadar Total Fenolik, Flavonoid, Tanin dan Kapasitas Antioksidan Ekstrak Etanol Kulit Kayu Manis Asal Bogor

Abstract

Kayu manis (Cinnamomum burmannii) merupakan tanaman herbal yang memiliki kandungan metabolit sekunder yang berperan sebagai antioksidan alami dalam menangkal radikal bebas. Penelitian ini bertujuan mengukur kadar total fenolik, flavonoid, tanin serta kapasitas antioksidan ekstrak etanol kulit kayu manis yang berasal dari Bogor. Ekstraksi dilakukan dengan metode maserasi dan dipekatkan dengan rotary evaporator. Pengukuran kadar total fenolik dan tanin dilakukan dengan metode Folin-Ciocalteu. Kadar total flavonoid diukur dengan metode aluminium klorida. Kapasitas antioksidan diukur menggunakan metode DPPH, FRAP, dan TBA. Hasil penelitian menunjukkan kadar total fenolik, flavonoid, dan tanin berturut-turut sebesar 299,05 ± 1,18 mg GAE/g ekstrak, 42,62 ± 1,41 mg QE/g ekstrak, dan 178,53 ± 6,74 mg TAE/g ekstrak. Kapasitas antioksidan yang diperoleh melalui metode DPPH dan FRAP berturut-turut adalah 875,06 ± 1,22 µmol TE/g ekstrak dan 2031,11 ± 5,55 µmol TE/g ekstrak dengan rata-rata persen inhibisi peroksidasi lipid MDA sebesar 80,15 ± 0,002.

Cinnamon bark (Cinnamomum burmannii) is an herbal plant that serves as a source of secondary metabolites, acting as a natural antioxidant to combat free radicals. This study aimed to determine the total phenolic, flavonoid, tannin content, as well as the antioxidant capacity of ethanol extract from cinnamon bark sourced from Bogor. Extraction was performed using the maceration method, followed by concentration with a rotary evaporator. The total phenolic and tannin content was measured using the Folin-Ciocalteu method, while the total flavonoid content was determined using the aluminium chloride method. Antioxidant capacity was evaluated using the DPPH, FRAP, and TBA assays. The results showed that the total phenolic, flavonoid, and tannin content were 299,05 ± 1,18 mg GAE/g extract, 42,62 ± 1,41 mg QE/g extract, and 178,53 ± 6,74 mg TAE/g extract, respectively. The antioxidant capacity measured by the DPPH and FRAP methods was 875,06 ± 1,22 µmol TE/g extract and 2031,11 ± 5,55 µmol TE/g extract, respectively, with an average lipid peroxidation (MDA) inhibition percentage of 80,15 ± 0,002.