Efektivitas In Ovo Feeding Ekstrak Daun Pegagan (Centella asiatica) Terhadap Profil Darah Anak Ayam Sentul

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh in ovo feeding ekstrak daun Pegagan (Centella asiatica) terhadap profil darah anak ayam Sentul. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) terdiri atas 5 perlakuan dan 5 ulangan. Setiap ulangan menggunakan 10 butir telur fertil ayam Sentul. Perlakuan terdiri atas P0= telur tanpa injeksi, P1= telur diinjeksi 0,5 ml PBS (Phosphate Buffered Salline), P2 = telur diinjeksi 0,5 ml PBS + 1 mg ekstrak daun Pegagan, P3 = telur diinjeksi 0,5 ml PBS + 2 mg ekstrak daun Pegagan, P4 = telur diinjeksi 0,5 ml PBS + 4 mg ekstrak daun Pegagan. Hasil penelitian ini menunjukkan bahwa in ovo feeding ekstrak daun Pegagan tidak berpengaruh nyata (P>0,05) terhadap jumlah hemoglobin, hematokrit, eritrosit, diferensiasi leukosit dan rasio HL. Perlakuan memberikan pengaruh nyata (P<0,05) terhadap jumlah leukosit. Kesimpulan penelitian ini adalah perlakuan in ovo feeding dengan ekstrak daun Pegagan pada level hingga 4 mg tidak mempengaruhi level hemoglobin, hematokrit, eritrosit, diferensiasi leukosit, dan rasio HL, namun mampu meningkatkan jumlah leukosit dalam meningkatkan imunitas anak ayam Sentul.

This study aims to determine the effect of in ovo feeding with Pegagan leaf extract (Centella asiatica) on the blood profile of Sentul chicks. This study used a Completely Randomized Design (CRD) with 5 treatments and 5 replicates. Each replicate using of 10 fertile eggs of Sentul chicken. Treatment consisted of P0 = uninjection egg, P1 = injected egg of 0,5 ml PBS (Phosphate Buffered Salline), P2 = injected egg of 0,5 ml PBS + 1mg Pegagan leaf extract, P3 = injected egg of 0,5 ml PBS + 2 mg Pegagan leaf extract, P4 = injected egg of 0,5 ml PBS + 4 mg Pegagan leaf extract. The results of this study showed that in ovo feeding of Pegagan leaf extract had no significant effect (P>0,05) on the amount of hemoglobin, hematocrit, erythrocytes, leukocyte differentiation and the HL ratio. The treatment had a significant effect (P<0,05) on the number of leukocytes. Its concluded that in ovo feeding treatment with Pegagan leaf extract at a levels up to 4 mg did not affect the levels of hemoglobin, hematocrit, erythrocytes, leukocyte differentiation, and the HL ratio, but was able to increase the number of leukocytes in increasing the immunity of Sentul chicks.