Pengaruh Induksi Mutasi Kimia Tanaman Portulaca Oleracea Dengan Ethyl Methane Sulfonate (EMS) Terhadap Kapasitas Antioksidan

Abstract

Kandungan total flavonoid, fenolik dan kapasitas antioksidan pada tanaman Portulaca oleracea L hasil mutasi dengan EMS. Penelitian ini bertujuan menetukan karakteristik fitokimia dan kapasitas antioksidan pada Portulaca oleracea L yang telah diinduksi EMS dengan konsetrasi dan lama perendaman optimum. Krokot diekstraksi dengan metode Microwave Assisted Extraction dan pelarut metanol 96%. Kadar total fenolik dihitung dengan metode Folin-Ciocalteu, kadar total flavonoid dengan metode AlCl3, serta kapasitas antioksidan dengan metode DPPH dan FRAP. Kadar total fenolik tertinggi terdapat pada sampel dengan konsentrasi EMS 750 dan waktu perendaman 4 jam 0.7336 mg GAE/g BB. Kadar total flavonoid tertinggi terdapat pada sampel dengan konsentrasi 750 dan waktu perendaman 8 jam 2,127 mg QE/g BB.

The total flavonoids, phenolics and antioxidant capacity in Portulaca oleracea L plants mutated with EMS are unknown. This study aimed to determine the phytochemical characteristics and antioxidant capacity in EMS-induced Portulaca oleracea L with optimum concentration and soaking time. Purslane was extracted by Microwave Assisted Extraction method and 96% methanol solvent. Total phenolic content was calculated by Folin-Ciocalteu method, total flavonoid content by AlCl3 method, and antioxidant capacity by DPPH and FRAP methods. The highest total phenolic content was found in the sample with EMS concentration 750 and immersion time 4 hours 0.7336 mg GAE/g WW. The highest total flavonoid content was found in the sample with an EMS concentration of 750 and an immersion time of 8 hours 2.127 mg QE/g WW.