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Title: | Validasi Metode Penentuan Kadar Timbal dalam Kecap Manis dengan Destruksi Basah Secara Spektrofotometri Serapan Atom Nyala |
Other Titles: | Validation Method for Determination Lead Levels in Sweet Soy Sauce with Wet Digestion using Flame Atomic Absorption Spectrophotometry |
Authors: | Supardan, Atep Dian Tiyagita, Isykariema Putri |
Issue Date: | 2024 |
Publisher: | IPB University |
Abstract: | Keberadaan logam timbal (Pb) dalam kecap perlu dilakukan pengawasan
mengingat akibat bahayanya logam tersebut jika masuk ke dalam tubuh dengan
jumlah berlebih. Menurut SNI 7387:2009 batas kadar timbal yang diperbolehkan
dalam kecap adalah sebesar 1,0 mg/kg. Penentuan logam timbal dilakukan
menggunakan metode spektrofotometri serapan atom (SSA) melalui proses
preparasi metode destruksi basah. Metode destruksi basah dilakukan dengan
menggunakan HNO3 65% dan HClO4 60% yang mengacu pada APHA 2023.
Sampel yang telah didestruksi kemudian diukur menggunakan spektrofotometer
serapan atom nyala Perkin Elmer 300 sebanyak 7 kali. Hasil penelitian
menunjukkan nilai Linearitas sebesar 0,999, nilai LD 0,0148 mg/L, nilai LK 0,0447
mg/L, nilai repeatabilitas 2,8328%, nilai %perolehan kembali sebesar 100,35%.
Analisis statistika pada uji ketahanan menunjukkan hasil f-hitung < f-tabel dan
analisis statistika pada uji ketangguhan menunjukkan hasil t-hitung < t-tabel. Nilai
seluruh parameter uji yang didapat ini telah memenuhi persyaratan yang ditentukan. The Presence of lead (Pb) in soy sauce needs to be monitored due to the harmful effects of this metal when ingested in excessive amounts. According to SNI 7387:2009, the permissible lead content in soy sauce is 1,0 mg/kg. The determination of lead metal is carried out using the atomic absorption spectrophotometry (AAS) method through a wet digestion preparation process. The wet digestion method is performed using 65% HNO3 dan 60% HClO4, referring to APHA 2023. The digested samples are then measured using a Perkin Elmer 300 flame atomic absorption spectrophotometer seven times. The result of the study shows a linearity value of 0.999, LOD of 0.0148 mg/L, LOQ of 0.0447 mg/L, repeatability value of 2.8328%, and a recovery rate of 100.35%. Statistical analysis of the robustness test shows f-calculated < f-table and statistical analysis of the ruggedness shows t-calculated < t-table. The values of all the obtained test parameters meet the specified requirements. |
URI: | http://repository.ipb.ac.id/handle/123456789/157113 |
Appears in Collections: | UT - Chemistry Analysis |
Files in This Item:
File | Description | Size | Format | |
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cover_J0312201058_83aca9c90f514b69aaa751c129d251d2.pdf | Cover | 327.89 kB | Adobe PDF | View/Open |
fulltext_J0312201058_30545cc126de407694342a71dd749581.pdf Restricted Access | Fulltext | 864.38 kB | Adobe PDF | View/Open |
lampiran_J0312201058_4cc2b149afaa442f861a6f92d36df412.pdf Restricted Access | Lampiran | 363.95 kB | Adobe PDF | View/Open |
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