Aktivitas Antimikroba dan Antihipertensi Hidrolisat Protein Putih Telur Ayam IPB D1 Hasil Hidrolisis Enzim Pepsin

Abstract

Telur merupakan pangan yang memiliki fungsi sebagai sumber protein hewani yang sering dikonsumsi karena memiliki kandungan asam amino essensial yang lengkap. Penelitian ini bertujuan menganalisis aktivitas antimikroba dan antihipertensi dari hidrolisat protein putih telur hasil hidrolisis enzim pepsin. Hidrolisis dilakukan dengan waktu inkubasi (0, 6, 12 jam) dan konsentrasi pepsin yang berbeda (0%, 0,5%, 1%). Telur ayam IPB D1 memiliki nilai HU 80,75 dengan grade AA dengan konsentrasi protein 96,70 mg/mL. Pengukuran derajat hidrolisis terbesar berada di angka 19,70% dengan masa inkubasi 12 jam dan konsentrasi enzim 1%. Uji aktivitas antimikroba menunjukkan hidrolisis protein dapat meningkatkan nilai aktivitas antimikroba yang diuji terhadap bakteri Escherichia coli, Salmonella, Bacillus cereus dan Staphylococcus aureus. Hasil pengujian menunjukkan bahwa proses hidrolisis dapat berpengaruh nyata (P<0,05) terhadap antihipertensi. Uji aktivitas antihipertensi, perlakuan P0 memiliki nilai rata-rata 61,38%, sedangkan perlakuan P1 memiliki rata-rata 33,24%.

Egg has a function as a source of animal protein that is often consumed because it contains complete essential amino acids. This study aims to analyze the antimicrobial and antihypertensive activity of egg white protein hydrolysate from pepsin enzyme hydrolysis. Hydrolysis was carried out with incubation time (0, 6, 12 hours) and different pepsin concentrations (0%, 0.5%, 1%). IPB D1 chicken eggs had a HU value of 80,75 with grade AA with a protein concentration of 96,70 mg/mL. The largest degree of hydrolysis measurement was 19,70% with an incubation period of 12 hours and 1% enzyme concentration. Antimicrobial activity test showed that protein hydrolysis can increase the value of antimicrobial activity tested against Escherichia coli, Salmonella, Bacillus cereus and Staphylococcus aureus bacteria. The test results show that the hydrolysis process can have a significant effect (P<0,05) on antihypertension. Antihypertensive activity test, P0 treatment has an average value of 61.38%, while P1 treatment has an average of 33.24%.