Aktivitas Antioksidan Fraksi Madu Apis cerana dan Potensinya sebagai Serum Wajah

Abstract

Apis cerana merupakan salah satu lebah penghasil madu asal Asia yang banyak dibudidayakan di Indonesia. Madu Apis cerana telah banyak diteliti memiliki aktivitas antioksidan. Penelitian ini bertujuan menganalisis aktivitas antioksidan sediaan serum wajah dari fraksi madu multiflora Apis cerana. Madu difraksinasi dengan corong pisah menggunakan pelarut akuades, n-heksana, dan etil asetat. Fraksi kemudian dilakukan pengukuran total fenolik, flavonoid, antioksidan DPPH (2,2-difenil-1-pikrilhidrazil), AEAC (Ascorbic acid Equivalent Antioxidant Capacity), dan PFRAP (Potassium Ferric Reducing Power). Hasil penelitian menunjukkan fraksi etil asetat merupakan sampel terbaik berdasarkan total fenolik (1972 ± 62,8 mg GAE/kg sampel), total flavonoid (1904,8 ± 9,2 mg QE/kg sampel), antioksidan DPPH IC50 (49204,1 ± 2763,6 mg/mL), AEAC (251,50 ± 8,83 mg AEAC/kg sampel) dan PFRAP (2445,6 ± 82,8 mg AAE/kg sampel). Fraksi etil asetat dipilih untuk diformulasikan menjadi serum wajah berdasarkan IC50. Serum wajah berhasil diformulasikan dan memiliki aktivitas antioksidan DPPH %inhibisi 15,84 ±1,27% serta antioksidan PFRAP 0,697 ± 0,049 AU.

Apis cerana is one of the honey-producing bee from Asia that widely cultivated in Indonesia. Apis cerana honey has been researched for its antioxidant activity. This study aims to analyze the antioxidant activity of facial serum made from fraction of Apis cerana multiflora honey. Honey was fractionated using distilled water, n-hexane, and ethyl acetate in a split funnel. The fractions were then evaluated for total phenolic content, flavonoid content, DPPH antioxidant activity (2,2-diphenyl-1-picrylhydrazyl), AEAC antioxidant (Ascorbic Acid Equivalent Antioxidant Capacity), and PFRAP antioxidant (Potassium Ferric Reducing Ability of Plasma). The results indicated that the ethyl acetate fraction was the most effective based on total phenolic content (1972 ± 62,8 mg GAE/kg sample), total flavonoid content (1904,8 ± 9,2 mg QE/kg sample), DPPH antioxidant IC50 (49204,1 ± 2763,6 mg/mL), AEAC (251,50 ± 8,83 mg AEAC/kg sample), and PFRAP (2445,6 ± 82,8 mg AAE/kg sample). The ethyl acetate fraction was selected for formulating the facial serum based on its IC50 value. The facial serum was successfully formulated and demonstrated DPPH antioxidant 15,84 ± 1,27% inhibition and PFRAP antioxidant activity 0,697 ± 0,049 AU.