Pemberian Kombinasi Ekstrak Daun Kelor dan EM-4 dalam Air Minum terhadap Penurunan Kadar Amonia Ekskreta Puyuh Petelur

Abstract

Penelitian ini bertujuan menganalisis efek pemberian ekstrak daun kelor dan EM-4 terhadap penurunan kadar amonia ekskreta puyuh petelur serta hubungan antar peubah yang diamati. Sebanyak 150 ekor puyuh betina dipelihara dalam rancangan acak lengkap (RAL) dengan 3 perlakuan dan 5 ulangan. Perlakuan terdiri dari P0 = Tanpa ekstrak daun kelor dan EM-4 dalam air minum ekor-1 hari-1 (kontrol), P1 = Penambahan 2 ml ekstrak daun kelor dan EM-4 ekor-1 hari-1, P2 = Penambahan 4 ml ekstrak daun kelor dan EM-4 ekor-1 hari-1. Peubah yang diukur meliputi kadar amonia (NH3), protein kasar, nitrogen, kadar air, dan pH ekskreta. Data dianalisis dengan uji ragam (ANOVA) dan uji Duncan bila hasil ANOVA signifikan, serta analisis korelasi Pearson untuk hubungan antar peubah. Hasil menunjukkan bahwa kombinasi ekstrak dan EM-4 tidak berpengaruh signifikan (P>0,05) terhadap kadar amonia (NH3), kadar air, dan pH ekskreta. Kombinasi ini secara signifikan menurunkan (P<0,05) kadar protein kasar dan nitrogen ekskreta pada perlakuan P1. Amonia ekskreta berkorelasi sangat kuat dengan kadar air dan pH ekskreta (r = 0,87 dan r = 0,92). Kadar protein kasar berkorelasi sempurna dengan kadar nitrogen ekskreta (r = 1,00), dan kadar air ekskreta berkorelasi sangat kuat dengan pH ekskreta (r = 0,96).

This study aimed to analyze the effects of providing Moringa leaf extract and EM-4 on the reduction of ammonia levels in layer quail excreta and the relationship between the observed variables. A total of 150 female quails were raised in a completely randomized design (CRD) with 3 treatments and 5 replicates. The treatments were: P0 = Moringa leaf extract and EM-4 in drinking water bird-1 day-1 (control), P1 = Addition of 2 ml of Moringa leaf extract and EM-4 in drinking water bird-1 day-1, and P2 = Addition of 4 ml of Moringa leaf extract and EM-4 in drinking water bird-1 day-1. Variables measured included ammonia (NH3) content, crude protein, nitrogen, water content, and excreta pH. Data were analyzed using analysis of variance (ANOVA) and Duncan's test if ANOVA results were significant, and Pearson correlation analysis for the relationship between variables. Results showed the combination of extract and EM-4 didn’t significantly affect (P>0.05) ammonia (NH3) content, water content, and excreta pH. This combination significantly decreased (P<0.05) crude protein and nitrogen content of excreta in treatment P1. Excreta ammonia was strongly correlated with water content and excreta pH (r = 0.87 and r = 0.92). Crude protein content was perfectly correlated with nitrogen content of excreta (r = 1.00), and water content of excreta was strongly correlated with excreta pH (r = 0.96).