Aplikasi Suplemen Nanokalsium-Vitamin D atau Enzim Protease terhadap Karakteristik Fermentasi Rumen Domba Penggemukan

Abstract

Penelitian ini bertujuan untuk menganalisis karakteristik fermentasi rumen pada domba penggemukan dengan suplementasi nanokalsium-vitamin D, enzim bromelin atau enzim papain. Ternak yang digunakan adalah 12 ekor domba ekor tipis dengan bobot badan awal 13,8 ± 1,36 kg. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) yang terdiri atas empat perlakuan yaitu P0 (rumput pakchong 30% + konsentrat 70%) sebagai kontrol, P1 (P0 + nanokalsium-vitamin D 5,06 g kgBB-1 hari-1), P2 (P0 + enzim bromelin 108 mg kgBB-1 hari-1), dan P3 (P0 + enzim papain 108 mg kgBB-1 hari-1). Peubah yang diamati meliputi nilai pH, populasi protozoa, populasi total bakteri, NH3, VFA total, VFA parsial, produksi CH4, kadar kalsium, dan kadar allantoin rumen. Hasil penelitian menunjukkan bahwa perlakuan nanokalsium-vitamin D, enzim bromelin, dan enzim papain tidak berpengaruh nyata (P>0,05) terhadap nilai pH, populasi protozoa, populasi total bakteri, konsentrasi NH3, VFA total, VFA parsial, produksi CH4, kadar allantoin, namun berpengaruh nyata (P<0,05) terhadap peningkatan kadar kalsium dalam rumen. Dapat disimpulkan bahwa pemberian suplementasi nanokalsium-vitamin D, enzim bromelin, atau enzim papain pada domba tidak mengganggu aktivitas fermentasi di dalam rumen.

This study aimed to analyze the characteristics of rumen fermentation in fattening sheep with supplementation of nanocalcium-vitamin D, bromelain enzyme, and papain enzyme. A total of 12 thin-tailed sheep with an initial body weight of 13.8 ± 1.36 kg was used in this study. This study used a Completely Randomized Block (CRBD) consisting of four treatments, namely P0 (pakchong grass 30% + consentrate 70%) as control, P1 (P0 + nanocalcium-vitamin D 5.06 g kgBW-1 day-1), P2 (P0 + bromelain enzyme 108 mg kgBW-1 day-1), and P3 (P0 + papain enzyme 108 mg kgBW-1 day-1). Parameters measured included pH value, protozoa population, total bacteria population, NH3, total VFA, partial VFA, CH4 production, calcium level, and allantoin level in the rumen. The results showed that the treatment of nanocalcium-vitamin D, bromelain enzyme, or papain enzyme had no significant effect (P>0.05) on pH value, protozoa population, total bacteria population, NH3 concentration, total VFA, partial VFA, CH4 production, and allantoin levels. Still, they significantly increased (P<0.05) the rumen calcium levels. Supplementation of nanocalcium-vitamin D, bromelain enzyme, or papain enzyme in sheep does not interfere with fermentation activity in the rumen.