Resistansi Antibiotik pada Isolat Escherichia coli dari Daging Mi Ayam di Lingkar Kampus Dramaga IPB University

Abstract

Mi ayam merupakan salah satu pangan asal hewan yang berpotensi terkontaminasi bakteri Escherichia coli (E. coli) resistan terhadap antibiotik. Bakteri ini mengancam kesehatan masyarakat karena sulit mengendalikan penyebaran bakteri penyebab infeksi. Tujuan penelitian ini menentukan tingkat resistansi antibiotik isolat E. coli dari daging ayam cincang mi ayam yang dijual di lingkar Kampus Dramaga, IPB University. Pengujian resistansi menggunakan metode Kirby-Bauer disk diffusion dan mengacu pada Clinical Laboratory Standard Institute 2023. Sebanyak 43 isolat teridentifikasi E. coli, tingkat resistansi E. coli diperoleh terhadap ampisilin (49%), tetrasiklin (44,2%), sulfametoksazol trimetoprim (33%), kloramfenikol (23,3%), streptomisin (16,3%), dan sefotaksim (9,3%). Selain itu, isolat E. coli menunjukkan resistansi tingkat intermediet pada antibiotik streptomisin (35%) dan sefotaksim (35%). Isolat bakteri E. coli masih sensitif terhadap kloramfenikol (72%), sulfametoksazol-trimetoprim (65,1%), tetrasiklin (56%), sefotaksim (56%), streptomisin (49%), dan ampisilin (35%). Terdapat isolat E. coli bersifat multidrug-resistance dengan pola ampisilin streptomisin-tetrasiklin-sefotaksim-sulfametoksazol trimetoprim-kloramfenikol. Tingkat resistansi antibiotik yang tinggi pada isolat E. coli memicu masalah kesehatan masyarakat karena bakteri yang resistan dapat menyebar ke tubuh melalui konsumsi makanan yang terkontaminasi, menimbulkan risiko infeksi yang lebih besar.

Chicken noodles are one of the animal-derived foods that have the potential to be contaminated with antibiotic-resistant Escherichia coli (E. coli) bacteria. These bacteria threaten public health because it is difficult to control the spread of bacteria that cause infections. The purpose of this study was to determine the level of antibiotic resistance of E. coli isolates from minced chicken meat chicken noodles sold around the Dramaga Campus, IPB University. Resistance testing uses the Kirby-Bauer disk diffusion method and refers to the Clinical Laboratory Standard Institute 2023. A total of 43 E. coli isolates were identified, the resistance level of E. coli was obtained against ampicillin (49%), tetracycline (44,2%), sulfamethoxazole-trimethoprim (33%), chloramphenicol (23,3%). streptomycin (16,3%), and cefotaxime (9,3%). In addition, E. coli isolates showed intermediate level resistance to streptomycin (35%) and cefotaxime (35%) antibiotics. E. coli isolates were still sensitive to chloramphenicol (72%), sulfamethoxazole- trimethoprim (65,1%), tetracycline (56%), cefotaxime (56%), streptomycin (49%), and ampicillin (35%). There were multidrug-resistant E. coli isolates with ampicillin-streptomycin-tetracycline-cephotaxime-sulfamethoxazole trimethoprim chloramphenicol pattern. The high level of antibiotic resistance in E. coli isolates triggers public health concerns as resistant bacteria can spread to the body through the consumption of contaminated food, posing a greater risk of infection.