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Title: | Preparasi Antigen Virus Newcastle Disease untuk Reagen Uji Hemaglutinasi Inhibisi |
Other Titles: | |
Authors: | Poetri, Okti Nadia Wulansari, Retno Salsabila, Syaharani |
Issue Date: | 2024 |
Publisher: | IPB University |
Abstract: | Preparasi antigen Newcastle Disease (ND) secara mandiri dapat dilakukan untuk memenuhi kebutuhan reagen uji pada laboratorium privat dengan biaya yang lebih ekonomis. Penelitian ini bertujuan melakukan preparasi antigen ND yang sebanding dengan antigen ND komersial untuk digunakan sebagai reagen uji hemaglutinasi inhibisi (HI). Preparasi antigen ND menggunakan buffer neutral formalin (BNF) sebagai bahan penginaktivasi virus dan gliserin sebagai bahan pengawet. Validasi antigen dilakukan dengan melakukan uji HI terhadap 22 sampel serum darah ayam menggunakan antigen ND mandiri dan antigen ND komersial Pusat Veteriner Farma (Pusvetma) secara paralel. Data titer antibodi dianalisis
dengan Analysis of Variant (ANOVA), uji sensitivitas (Se), uji spesifisitas (Sp), dan uji Kappa (K). Hasil analisis data menunjukkan bahwa rataan titer antibodi HI menggunakan antigen ND mandiri dan antigen ND komersial Pusvetma tidak berbeda nyata (p>0.05), nilai Se 100%, nilai Sp 100%, serta nilai K mencapai 1. Hasil penelitian mengindikasikan bahwa preparasi antigen ND yang telah dilakukan menghasilkan antigen ND yang sebanding dengan antigen ND komersial Pusvetma, sehingga dapat digunakan sebagai reagen pada uji HI. Newcastle Disease (ND) antigen can be prepared autonomously for private laboratories to be more economical. This study aims to prepare ND antigen which is comparable to the commercial ND antigen as a reagent for the hemagglutination inhibition (HI) test. ND antigen preparation used buffered neutral formalin (BNF) as a virus inactivating agent and glycerol as a preservative. Antigen validation was carried out by conducting an HI test on 22 chicken blood serum samples using the self-made ND antigen and the commercial Pusat Veteriner Farma (Pusvetma) ND antigen in parallel. Antibody titer data were analyzed using ANOVA, sensitivity test (Se), specificity test (Sp), and Kappa test (K). The results of data analysis showed that the average HI antibody titer using the self-made ND antigen and commercial Pusvetma ND antigen has no significant difference (p>0.05), the Se and Sp values were 100%, and the K value reached 1. The results of the study indicated that ND antigen preparation using this method produced ND antigen which is comparable to the commercial ND antigen (Pusvetma), so it can be used as a reagent in the HI test. |
URI: | http://repository.ipb.ac.id/handle/123456789/153615 |
Appears in Collections: | UT - Anatomy, Phisiology and Pharmacology |
Files in This Item:
File | Description | Size | Format | |
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cover_B0401201015_42ce00c63e424c74a9ecdd7933fcb101.pdf | Cover | 518.61 kB | Adobe PDF | View/Open |
fulltext_B0401201015_ed6841a4e1124a9c9df0037480dc5079.pdf Restricted Access | Fulltext | 638.67 kB | Adobe PDF | View/Open |
lampiran_B0401201015_4881e1a3cf5c47c6b8ad11009cec8060.pdf Restricted Access | Lampiran | 251.47 kB | Adobe PDF | View/Open |
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