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http://repository.ipb.ac.id/handle/123456789/122657
Title: | Pengembangan Metode Uji Cepat Viabilitas dan Vigor Benih Jagung (Zea mays L.) dengan Urine Sugar Analysis Paper |
Other Titles: | Development of Rapid Viability and Vigor Test Methods for Corn (Zea mays L.) Seeds Using Urine Sugar Analysis Paper |
Authors: | Suhartanto, M. Rahmad Ilyas, Satriyas Fitri, Risa Destiana |
Issue Date: | 2023 |
Publisher: | IPB University |
Abstract: | Urine sugar analysis paper (USAP) telah digunakan untuk uji cepat
viabilitas dan vigor benih kedelai berdasarkan terdeteksinya bocoran protein di
dalam air rendaman benih. Penelitian bertujuan mengembangkan metode uji cepat
viabilitas dan vigor benih jagung menggunakan USAP dengan mendeteksi
bocoran glukosa, protein, dan perubahan pH pada berbagai tingkat viabilitas dan
vigor. Tahap pertama penelitian ini adalah pembuatan lot benih dengan tiga bulan
penyimpanan, disusun menggunakan rancangan acak lengkap (RAL) satu faktor
yaitu kemasan benih (plastik polypropylene, plastik polyethylene, kertas amplop
coklat, dan tanpa kemasan). Tahap kedua adalah uji kebocoran glukosa, protein
dan perubahan pH menggunakan USAP. Percobaan menggunakan RAL satu
faktor, 12 kombinasi perlakuan yaitu kondisi benih (utuh, dibelah dua, dan
dihaluskan) dengan lama perendaman (0, 6, 9, dan 12 jam). Hasil tahap pertama
dan tahap kedua dianalisis menggunakan analisis regresi linier sederhana dan uji
korelasi untuk mengetahui hubungan antara keduanya. Hasil penelitian
menunjukkan USAP mampu mendeteksi bocoran glukosa pada perlakuan benih
dibelah dua dan dihaluskan pada semua lama perendaman, namun tidak dapat
membedakan tingkat viabilitas dan vigor benih. Indikator bocoran protein dan
perubahan pH tidak terdeteksi sehingga tidak dapat membedakan tingkat viabilitas
dan vigor benih. Urine sugar analysis paper (USAP) has been used to test the viability and vigor of soybean seeds based on detection of protein leakage in the seed’s soaking water. The research aimed to develop a rapid viability and vigor test method for corn seeds using USAP by detecting leaks of glucose, protein and changes in pH at various levels of viability and vigor. The first stage of this research was making seed lots with three months of storage, arranged using a completely randomized design (CRD) with one factor, namely seed packaging (polypropylene plastic, polyethylene plastic, brown envelope paper, and without packaging). The second stage was testing for glucose and protein leakage and changes in pH using USAP. The experiment used a CRD with one factor, consisted of 12 treatment combinations, namely seed conditions (intact, halved, and crushed) with different soaking durations (0, 6, 9, and 12 hours). The results of the first and second stages were analyzed using simple linear regression analysis and correlation tests to determine the relationship between them. The results showed that USAP could detect glucose leakage in the halved and crushed seed treatments at all soaking durations but could not differentiate between seed viability and vigor levels. The indicators of protein leakage and pH changes were not detected thus could not distinguish seed viability and vigor levels. |
URI: | http://repository.ipb.ac.id/handle/123456789/122657 |
Appears in Collections: | UT - Agronomy and Horticulture |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
COVER.pdf Restricted Access | Cover | 992.06 kB | Adobe PDF | View/Open |
FULL TEKS.pdf Restricted Access | Full Text | 1.35 MB | Adobe PDF | View/Open |
LAMPIRAN.pdf Restricted Access | Lampiran | 690.26 kB | Adobe PDF | View/Open |
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