Environmental Biosensor Development for Organochlorine Pesticides Detection based on Carbon Paste Electrode Modified by Nanoparticle

Abstract

Increasing of activity in the agricultural and industrial sectors become undivided part of life. Besides positive impact for humans, these activities also had negative impact in the form of environmental damage caused by contamination. In the agricultural sector, farmer used various types of pesticides to increase quality and productivity of agricultural products. However, the use of pesticides also give bad effect to the environment and human life. Based on Stockholm Convention (2001), pesticides included into persistent organic pollutants (POPs), namely that is organic compounds that are resistant to environmental degradation through chemical, biological, and photocatalytical. Pesticide compound group of organochlorines was the most toxic of organophosphates or carbamates. Various methods have been developed and used as a analysis method in environmental monitoring. Generally the methods currently used for the identification of pesticide are the method of gas chromatography and high performance liquid chromatography. However, the methods that have been used is relatively expensive methods. These methods also cannot be used for direct identification of pesticide compounds in the environment. To solve that problem, biosensor technology become alternatively main selection. Important step in the development of biosensors is the process of modifying the electrode and immobilization of biological component. In this research was used nanoparticle zeolite as electrode modifier and immobilization matrix of biological component. Acetylcholinesterase enzyme (Ache) was used as biological component with acetylcholine chloride as substrate. Electrochemical measurement use cyclic voltammetry. Optimization of independently variables factors to obtain optimum immobilized Ache activity response th concentration, and the concentration of acetylcholine chloride.