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http://repository.ipb.ac.id/handle/123456789/119554
Title: | Intervensi Nikotin Terhadap Profil Lipid Ekspresi UCP-1 (Uncoupling Protein-1) Pada Monyet Ekor Panjang (Macaca fascicularis)Obes dengan Risiko Aterogenik |
Other Titles: | Lipid Profiles and UCP-1 Expression in Cynomolgus Monkey with Atherogenic Risk AfterNicotine Intervention |
Authors: | Sajuthi, Dondin Yusuf, Irawan Sulistiawati, Erni Mansjoer, Sri Supraptini Warongan, Anwar Wardy |
Issue Date: | 2012 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | Pola makan dengan tinggi kalori tanpa disertai dengan pengaturan keseimbangan
energi antarakalori yang dikonsumsi dan kalori yang dikeluarkan merupakan penyebab
dasarkelebihan berat badan yang mengarah ke obesitas. Kondisi ini sudah menjadi
masalah global terutama di negara-negara berkembang dan di negara-negara maju
olehkarena memiliki berbagai faktor resiko terhadap sindrom metabolik yang berkaitan
dengan penyakit jantung koroner (PJK), diabetes melitus, dan hipertensi.
Kelebihan berat badan pada sindrom metabolik dengan resiko tinggi
aterosklerosis (atherogenetic process) diidentifikasi melalui adanya peningkatan indeks
bobot badan (body mass index; BMI), lingkar pinggang atau rasio lingkar pinggang dan
pinggul. Jaringan adipos dikenal sebagai tempat penyimpanan triglserida (TG), dan
sebagai jaringan endokrin dinamik yang mengsekresikan free fatty acid (FAA) dan
berbagai protein seperti adiponectin dan adipokin. Adapun profi lipid yang penting untuk
mendiagnosis hiperlipoproteinemia yang mencakup ~lipidemia meliputi peubah
kolesterol totai trigliserida, LDL-kolesterol dan HDL-kolesterol. Jaringan adipose
diketahui pula sebagai organ endokrinaktif yang dapat mengsekresikan berbagai adipokin
dan salah satunya adiponektin berperan dalam metabolisme lipid dan karbohidrat dalam
sirkulasi darah dan berpotensi dalam sindrom metabolik. Obesityis an abnormal accumulation of body fat with increased risk of coroner heart disease atherosclerosis, indicated by hyperlipoproteinemia. There are many treatments to prevent metabolic syndrome related to obesity, particularly with atherosclerosis risk. The aims of this study were to obtain information of metabolic changes through lipid profile and body weight also to evaluate UCP-1 expression post nicotine intervention on cynomolgus monkey with atherogenic risk. Fifteen animals were divided into three groups, given isocaloric diets containing 1) beef tallow (as diet A); 2) beeftallow and egg yolk (as Diet B) and other primary materials, and 3) commercial monkey chow diet (as a control or as Diet C). These diets were given for one year to develop obesity, then continued for three months by additing nicotine in their diet at a dose of at least 0. 75 mg I kg body weight every 12 hours. Body weight and lipid profile were analyzed every months and brown adipose tissue was taken at necropsy for evaluating UCP-1 expression by using antibody fluoresence technique. This study used a complete randomized design (ANOV A) to analyzes body weight and lipid profile. There were significantly increased in total plasma cholesterolconcentration (p<0.01), especially for diet type B and triglyceride concentration was not significantly increased (p>0.01). Followed by significantly increased HDL and LDL level, however there was decreased in body weight. Evaluation of UCP expression in this study showed expression of UCP-1 in each diet group A, B and C. Group B showed to have higher expression of UCP-1 than the other groups. |
URI: | http://repository.ipb.ac.id/handle/123456789/119554 |
Appears in Collections: | DT - Multidiciplinary Program |
Files in This Item:
File | Description | Size | Format | |
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2012aww.pdf Restricted Access | Fullteks | 1.65 MB | Adobe PDF | View/Open |
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