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Title: | Analisis keragaman genetik dan sintasan sejumlah isolat bekteri fotosintetik anoksigenik asal laut |
Authors: | Suwanto, Antonius Suharto, Maggy T Meryandini, Anja Rusmana, Iman |
Issue Date: | 1997 |
Publisher: | Bogor Agricultural University (IPB) |
Abstract: | Eighty isolates of anoxygenic photosynthetic bacte- ria were isolated from 49 samples collected from a number of Indonesian seashores. All of the isolates showed similar color (i.e. photosynthetically in red to brownish red) when grown Sea Water Complete (SWC) medium. under illumination of tungsten light (± 200 lux). Many of the isolates were gram-negative, rod shape bacteria. However, genetic analysis employing Asel-schizotyping of 45 selected isolates indicated extensive genetic diversi- ty which apparently did not show any biogeographic relat- edness. A number their survival of selected isolates in sterile sea water were examined for supplemented with peptone (0,1 g/1) and yeast extract (0,02 g/1). All of the isolates demonstrated significant growth until 5 days of incubation and most of them maintained their viability up to 7 days of incubation. However, SKX121 is the only isolate which could maintain its viability up to 2 x 109 cell/ml after 7 days of incubation. From 47 isolates examined, 35 isolates were signifi- cantly more tolerant to Na2S (up to 100 µg/ml than isolate MW4, a reference isolate which has been known as sulphide tolerant anoxygenic photosynthetic bacteria. |
URI: | http://repository.ipb.ac.id/handle/123456789/119546 |
Appears in Collections: | MT - Mathematics and Natural Science |
Files in This Item:
File | Description | Size | Format | |
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1997IRU.pdf Restricted Access | Fulltext | 2.55 MB | Adobe PDF | View/Open |
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