Bioteknologi kultur sel ginjal primer sebagai kandidat bahan uji vaksin dan model in-vitro

Abstract

Cell culture is a cell population that can proliferate in-vitro, isolating from the original tissue or grown from existing cells. Monkey kidney cell cultures are the one source of cell culture that has an essential role in biomedical research. There is significant homology between the human and macaque genomes making these useful for cultivating human viruses, especially human enteroviruses, and growing vaccines. In this study, we developed cell cultures derived from the kidney of Macaca fascicularis (Mf) and validated its gene expression. The primary cultures have been successfully subcultured up to six passages, grew as monolayer and primarily epithelial-like in morphology; the cultured cells remained heterogeneous in phenotype and they expressed (CD155 and CD46) as viral receptors, cell morphology (CD24, Endosialin, and vWF), proliferation, also apoptosis markers (Ki67 and p53). These result indicate these cell culture have potentiality to be used as in-vitro model cells for vaccine development and bioactive compound.