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http://repository.ipb.ac.id/handle/123456789/115133
Title: | Seleksi Khamir Lokal Penghasil Selenium Organik Asal Feses Sapi |
Other Titles: | Selection of Local Yeasts Producing Organic Selenium from Cow Faeces for Feed Additives |
Authors: | Mutia, Rita Wijayanti, Indah Almira, Adelia Pratista |
Issue Date: | 2022 |
Publisher: | IPB University |
Abstract: | Selenium (Se) merupakan mikromineral esensial, Se berperan dalam
melindungi tubuh ternak dari paparan radikal bebas. Pemberian Se-organik dapat
meningkatkan ketersediaan mineral dalam tubuh ternak sehingga daya serapnya
tinggi. Khamir merupakan mikroorganisme yang berperan dalam menyerap dan
mengakumulasi Selenium. Pemberian mineral dalam bentuk organik lebih efektif
dibandingkan mineral anorganik sehingga dapat dilakukan penggantian pada
mineral anorganik konvensional. Penelitian ini bertujuan untuk mengidentifikasi
dan menyeleksi khamir dari isolat lokal yang berasal dari feses sapi sebagai
penghasil selenium organik. Isolat dari kotoran sapi diseleksi dan diidentifikasi
dengan uji mikroskopis, makroskopis, dan biokimia untuk menentukan genus
isolat. Didapatkan empat isolat dengan kode FS 1, FS 3, FS 5, dan FS 6 dengan
dugaan kuat isolat khamir berturut-turut yaitu genus Saccharomyces, Hansenula,
Torulaspora, dan Candida. Isolat terpilih diberi perlakuan dengan penambahan
natrium selenit (Na2SeO3) sebanyak 30 ppm, 45 ppm, dan 60 ppm yang kemudian
dihitung menggunakan perhitungan Total Plate Counting (TPC). Isolat dengan
nilai pertumbuhan terbaik yaitu FS 1 dengan jumlah koloni 1,53 x 1011 cfu mL
-1
pada media tumbuh dengan konsentrasi Se 30 ppm. Khamir FS1 merupakan genus
Saccharomyces yang dihitung kandungan Se-nya menggunakan metode GF-AAS
yaitu sebesar 0,975 mg L-1
. FS1 mampu tumbuh baik pada media yang
mengandung Se, Cu dan Zn hingga konsentrasi 60 ppm. Selenium (Se) is an essential micromineral, Se has a role in protecting the body of livestock from exposure to free radicals. Provision of Se-organic can increase the availability of minerals in the body of livestock so that absorption is high. Yeast is a microorganism that has a role in absorbing and accumulating Se. The provision of minerals is more effective than minerals inorganic so that it can be carried out on conventional inorganic minerals. This study aims to identify and select yeast from local isolates in the form of cow feces as the best producer of organic selenium. Isolates from cow feces were selected and identified by microscopic, macroscopic, and biochemical tests to determine the genus. Four isolates were obtained with codes FS 1, FS 3, FS 5, and FS 6 with a strong suspicion of yeast isolates consecutively, namely the genus Saccharomyces, Hansenula, Torulaspora, and Candida. The selected isolates were treated with the addition of sodium selenite (Na2SeO3) as much as 30 ppm, 45 ppm, and 60 ppm which were then counted using Total Plate Counting (TPC) calculations. The isolate with the best growth value was FS 1 with the number of colonies 1.53 x 1011 cfu mL-1 on growth media with a concentration of Se 30 ppm. FS1 is a genus of Saccharomyces whose Se content was calculated using the GF-AAS method, which was 0.975 mg L-1 . FS1 was able to grow well on media containing Se, Cu and Zn up to a concentration of 60 ppm. |
URI: | http://repository.ipb.ac.id/handle/123456789/115133 |
Appears in Collections: | UT - Nutrition Science and Feed Technology |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cover, Lembar Pengesahan, Prakata, Daftar Isi.pdf Restricted Access | Cover | 240.06 kB | Adobe PDF | View/Open |
D24180117_Adelia Pratista Almira.pdf Restricted Access | Fullteks | 510.81 kB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 369 kB | Adobe PDF | View/Open |
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