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Title: | Pengaruh Genotipe dan Putresin terhadap Androgenesis Terong (Solanum melongena L.) pada Media Solid Secara In Vitro |
Other Titles: | Effect of Genotype and Putrescine on Androgenesis of Eggplant (Solanum melongena L.) on Solid Media In Vitro |
Authors: | Purwoko, Bambang Sapta Dewi, Iswari Saraswati Supriadi |
Issue Date: | 2022 |
Publisher: | IPB University |
Abstract: | Kultur antera dapat mempercepat perolehan galur murni untuk program
pemuliaan tanaman. Namun, metode kultur antera terong dengan efisiensi tinggi
belum diperoleh. Genotipe memiliki pengaruh yang besar terhadap keberhasilan
androgenesis terong. Penambahan putresin ke kultur antera dapat meningkatkan
jumlah kalus, jumlah embrio, dan jumlah tanaman yang diregenerasikan. Tujuan
penelitian ini ialah untuk mendapatkan genotipe dan konsentrasi putresin yang
optimal pada media induksi kultur antera terong. Bahan yang digunakan ialah
kuncup bunga terong dari tanaman yang dibudidayakan di rumah kaca. Kultur
antera dilakukan dalam tiga tahap percobaan: induksi embrio, perkecambahan
embrio, dan regenerasi tanaman. Percobaan induksi embrio dilakukan dengan 10
ulangan menggunakan Rancangan Acak Lengkap (RAL) dengan dua faktor
(genotipe dan putresin). Faktor genotipe terdiri atas tiga taraf (Pertiwi, Provita, dan
Edlyn). Faktor putresin terdiri atas tiga taraf konsentrasi (0 mM, 1 mM, dan 3 mM).
Antera yang berhasil diinduksi menjadi embrio disubkultur pada media kecambah
selama 2 minggu, dan kecambah kemudian disubkultur pada media perakaran untuk
diregenerasi. Hasil penelitian menunjukkan bahwa genotipe Edlyn menghasilkan
embrio paling banyak, tetapi tidak berbeda nyata dengan genotipe Provita. Putresin
1 mM menghasilkan embrio paling banyak yang berbeda nyata dengan putresin 0
mM dan putresin 3 mM. Efisiensi kultur antera terbaik adalah genotipe Edlyn
dengan konsentrasi putresin 1 mM. Tidak terdapat interaksi antara genotipe dan
putresin pada semua peubah, kecuali pada antera menghasilkan kalus dan waktu
muncul embrio. Anther culture can accelerate the acquisition of pure lines for plant breeding programs. However, eggplant anther culture media with high efficiency has not been obtained. Genotype has a great influence on the success of eggplant androgenesis. The addition of putrescine to anther culture media can increase the number of calluses, the number of embryos, and the number of plants. The purpose of this study was to obtain the optimal genotype and concentration of putrescine in eggplant anther culture. The material used was obtained from eggplant flower buds of plants cultivated in a greenhouse. Anther culture was carried out in three experimental stages: embryo induction, embryo germination, and plant regeneration. The embryo induction experiment was carried out with 10 replications using a completely randomized design (CRD) with two factors (genotype and putrescine). The genotype factor consisted of three levels (Pertiwi, Provita, and Edlyn). Putrescine factor consisted of three concentration levels (0 mM, 1 mM, and 3 mM). Anthers that were successfully induced into embryos were subcultured to germination media for 2 weeks, and the germinated embryo were then subcultured to rooting media. The results showed that Edlyn produced the highest number of embryos, but it was not significantly different from the Provita. Putrescine 1 mM produced the highest number of embryos which was significantly different from putrescine 0 mM and putrescine 3 mM. The best anther culture efficiency was obtained in Edlyn genotype with a putrescine concentration of 1 mM. There was no interaction between genotype and putrescine in all variables, except in the anthers producing callus and embryo emergence time. |
URI: | http://repository.ipb.ac.id/handle/123456789/114850 |
Appears in Collections: | UT - Agronomy and Horticulture |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cover_Supriadi (A24180030).pdf Restricted Access | Cover | 368.4 kB | Adobe PDF | View/Open |
Full text_Supriadi (A24180030).pdf Restricted Access | Full text | 645.08 kB | Adobe PDF | View/Open |
Lampiran_Supriadi (A24180030).pdf Restricted Access | Lampiran | 260.29 kB | Adobe PDF | View/Open |
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