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Title: | Pengaruh Pemanasan Cacing Laut (Nereis sp.) Terinfeksi White Spot Syndrome Virus (WSSV) Terhadap Patogenitas Virus pada Udang Vaname (Litopenaeus vannamei) |
Other Titles: | Effect of Heat Sea Worms (Nereis sp.) Infected with White Spot Syndrome Virus (WSSV) on Virus Pathogenicity in Vannamei Shrimp (Litopenaeus vannamei) |
Authors: | Sukenda, Sukenda Nuryati, Sri Iqbal, Rizki Muhamad |
Issue Date: | 2022 |
Publisher: | PB University |
Abstract: | Cacing polychaeta merupakan organisme yang digunakan sebagai pakan alami induk udang vaname, cacing ini dapat menjadi salah satu pembawa (carrier) WSSV dan berpotensi menginfeksi secara horizontal ke udang. Maka dari itu, perlu dilakukan pengendalian, salah satunya melalui pemanasan cacing. Tujuan penelitian ini adalah menguji pemberian perlakuan panas dengan suhu yang berbeda terhadap cacing laut yang terinfeksi WSSV sebagai pakan udang vaname L. vannamei. Udang yang digunakan yaitu udang yang berukuran 13 g. Udang dipelihara selama 14 hari dengan pemberian pakan cacing polychaeta yang telah diberi perlakuan panas. Perlakuan yang diberikan yaitu kontrol negatif (K-), kontrol positif (K+), cacing laut terinfeksi WSSV diberi perlakuan panas 60 °C (A), perlakuan panas 75 °C (B), dan perlakuan panas 90 °C (C) selama 60 detik. Pemberian perlakuan panas dengan suhu 90 °C selama 60 detik pada cacing laut yang terinfeksi WSSV mampu memberikan hambatan infeksi pada udang ditunjukkan dengan hasil PCR negatif, tidak adanya badan inklusi pada histopatologi organ hepatopankreas, tidak adanya gejala bintik putih, serta gambaran darah yang lebih baik. Kesimpulan penelitian ini adalah pemanasan pada cacing yang terinfeksi WSSV dapat menghambat transmisi horizontal virus WSSV. Polychaeta worms are organisms that are used as natural food for vaname shrimp, these worms can be one of the carriers of WSSV and have the potential to infect horizontally shrimp. Therefore, it is necessary to control, one of which is by heating the worms. The purpose of this study was to test the application of heat treatment with different temperatures to marine worms infected with WSSV as food for white shrimp L. vannamei. Shrimp used is shrimp measuring 13 g. Shrimp were reared for 14 days by feeding polychaeta worms that had been heating treated. The treatments were negative control (K-), positive control (K+), marine worms infected with WSSV were given heat treatment at 60 °C (A), heat treatment at 75 °C (B), and heat treatment at 90 °C (C) for 60 seconds. The provision of heat treatment at a temperature of 90 °C for 60 seconds on marine worms infected with WSSV was able to inhibit infection in shrimp as indicated by negative PCR results, the absence of inclusion bodies in the histopathology of the hepatopancreas organ, the absence of symptoms of white spots, and a better blood picture. This study concludes that heating WSSV-infected worms can inhibit horizontal transmission of WSSV virus. |
URI: | http://repository.ipb.ac.id/handle/123456789/112097 |
Appears in Collections: | UT - Aquaculture |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cover.pdf Restricted Access | Cover | 444.27 kB | Adobe PDF | View/Open |
C14170043_Rizki Muhamad Iqbal.pdf Restricted Access | Fullteks | 854.72 kB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 647.15 kB | Adobe PDF | View/Open |
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