Utilisasi Black Soldier Fly Fase Prepupa pada Berbagai Pengolahan Biologis sebagai Agen Mitigasi Metana

Abstract

Tujuan dari penelitian ini adalah mengevaluasi pengolahan black soldier fly (BSF) fase prepupa secara biologis untuk menurunkan kadar kitin yang merupakan komponen serat kasar dan sebagai agen mitigasi metana secara in vitro. Penelitian ini menggunakan rancangan acak kelompok dengan 3 perlakuan dan 5 ulangan. Perlakuan pada penelitian ini adalah P0 (BSF fase prepupa delipidasi tanpa perlakuan), P1 (BSF fase prepupa fermentasi Lactobacillus plantarum), dan P2 (BSF fase prepupa fermentasi Aspergillus oryzae). Peubah yang diamati adalah hasil analisis proksimat, rendemen kitin, derajat deasetilasi, dan hasil analisis fermentasi kecernaan secara in vitro. Data dianalisis menggunakan analysis of variance dan bila berbeda nyata diuji lanjut dengan uji Duncan. Hasil penelitian menunjukkan pengaruh berbeda nyata (P<0,05) terhadap kecernaan bahan kering, kecernaan bahan organik, VFA, NH3, produksi gas ke 72 jam, a+b (produksi gas maksimum), dan CH4. BSF fase prepupa fermentasi dengan Aspergillus oryzae dapat menjadi agen mitigasi metana yang mampu menurunkan emisi gas metan sebesar 2% pada ruminansia, kadar kitin sebesar 3,28%, dan serat kasar 4,44%.

The aim of this study was to evaluate the biological processing of black soldier fly (BSF) in the prepupa phase to reduce levels of chitin which is a component of crude fiber and as a methane mitigation agent in vitro. This study used a randomized block design with 3 treatments and 5 replications. The treatments in this study were P0 (BSF prepupae delipidation without treatment), P1 (BSF prepupae fermented Lactobacillus plantarum), and P2 (BSF prepupae fermented Aspergillus oryzae). The observed variables were the results of proximate analysis, chitin yield, degree of deacetylation, and the results of in vitro digestibility analysis. The data were analyzed using analysis of variance and if they were significantly different, they were further tested with Duncan's test. The results showed a significantly different effect (P<0.05) on dry matter digestibility, organic matter digestibility, VFA, NH3, gas production for 72 hours, a+b (maximum gas production), and CH4. BSF prepupae fermentation phase with Aspergillus oryzae can be a methane mitigation agent that can reduce methane gas emissions by 2% in ruminants, chitin content by 3.28%, and crude fiber by 4.44%.