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Title: | Isolasi, Karakterisasi, dan Pengklonan Gen Inhibitor Invertase (sininh) dari Berbagai Varietas Tebu di Indonesia |
Other Titles: | Isolation, Characterization, and Cloning of Invertase Inhibitor Gene (sininh) from several Sugarcane Varieties in Indonesia |
Authors: | Santosa, Dwi Giyanto Firlandiana, Maulidi |
Issue Date: | 15-Jan-2022 |
Publisher: | IPB University |
Abstract: | Fragmen gen inhibitor invertase telah diisolasi dari berbagai tanaman yang
dibudidayakan, salah satunya adalah tebu. Laporan hasil penelitian ini akan
mengulas struktur gen inhibitor invertase (sininh) sepanjang 650 bp dari beberapa
varietas tebu di Indonesia diantaranya varietas PS 881, PS 882, PSJT 941, PS 862,
BL (Bulu Lawang), dan KK (Kidang Kencana). Selain itu, struktur primer, sifat
fisikokimia, struktur sekunder, struktur 3D, dan lokalisasi subseluler dari protein
Sininh diprediksi menggunakan bioinformatic tools. Informasi ini bermanfaat
untuk penelitian di masa depan, terutama untuk mengontrol akumulasi sukrosa pada
tingkat pasca-translasi pada tanaman tebu. Prediksi protein Sininh yang terdiri dari
124 - 145 residu memiliki karakteristik yang berbeda-beda. Potein Sininh yang
diprediksi dari varietas PS 882 dan BL memiliki potensi translokasi karena
ditemukan sinyal peptida pada urutan sekuens nya. Selain itu, ditemukan beberapa
residu Cys (sistein) pada urutan prediksi protein Sininh kedua varietas tersebut yang
terkait dengan pembentukan ikatan disulfida untuk stabilitas struktur protein.
Domain lestari tanaman yaitu invertase/pektin methylesterase inhibitor yang
ditemukan dalam prediksi protein Sininh varietas BL/Bulu Lawang berperan
menghambat aktivitas pectin methylesterase (PMEs) invertase dengan
pembentukan suatu kompleks. Adapun motif N-glycosylation juga ditemukan
dalam protein Sininh varietas PS 881, PSJT 941, PS 862, BL (Bulu Lawang), dan
KK (Kidang Kencana), yang terkait dengan potensi stabilitas dan interaksi dengan
protein lainnya. Selain itu, pada penelitian ini berhasil dilakukan pengklonan gen
inhibitor invertase (sininh) dari tebu varietas PS 862 pada bakteri Eschericia coli
DH5α. Studi ini membangun dasar untuk mempelajari aspek struktural dan
mekanisme inaktivasi invertase melalui protein penghambat (Sininh) pada tingkat
molekuler. Invertase inhibitor gene fragments have been isolated from various cultivated plants, one of which is sugarcane. This report will review the structure of the invertase inhibitor gene (sininh) at the 650 bp from multiple sugarcane varieties in Indonesia (PS 881, PS 882, PSJT 941, PS 862, BL/Bulu Lawang, KK/Kidang Kencana). Moreover, the primary structure, physicochemical properties, the secondary structure, 3D structure, and subcellular localization of Sininh protein were predicted utilizing bioinformatics tools. This information is beneficial for future research, especially to control sucrose accumulation at the post-translation level in sugarcane plants. The prediction of the Sininh protein consisting of 124 - 145 residues have different characteristics. The predicted Sininh protein sequences from PS 882 and BL varieties have the potency for translocation due to peptide signals. In addition, there are some Cys residues in both varieties associated with the formation of disulfide bonds for protein structure stability. Sustainable, functional conserved domain plant invertase/pectin methylesterase inhibitors found in the Sininh protein variety BL/Bulu Lawang where they inhibit the activity of pectin methylesterase (PMEs) invertase with a complex formation. N-glycosylation motifs are also found in the protein Sininh varieties PS 881, PSJT 941, PS 862, BL (Bulu Lawang), KK (Kidang Kencana), which is related to potential stability and interaction with other proteins. Moreover, this study was successfully cloned sininh gene from PS 862 variety in Eschericia coli DH5α. These studies build the foundation for studying the structural aspects and the mechanism of the inactivation of invertase via its inhibitory proteins (Sininh) at the molecular level. |
URI: | http://repository.ipb.ac.id/handle/123456789/110645 |
Appears in Collections: | MT - Agriculture |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cover, Lembar Pengesahan, Prakata, Daftar Isi.pdf Restricted Access | Cover | 793.61 kB | Adobe PDF | View/Open |
A154190071_Maulidi Firlandiana.pdf Restricted Access | Full teks | 2.51 MB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 1.04 MB | Adobe PDF | View/Open |
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