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Title: | Aktinobakteri Endofit Xylocarpus granatum: Eksistensi dan Aktivitas Antioksidan |
Other Titles: | Xylocarpus granatum Endophytic Actinobacteria: Presence and Activity of Antioxidants |
Authors: | Lestari, Yulin Batubara, Irmanida Ratte, Marina |
Issue Date: | Nov-2021 |
Publisher: | IPB University |
Abstract: | Xylocarpus granatum menghasilkan berbagai senyawa bioaktif dengan
aktivitas biologis yang beragam, salah satunya sebagai antioksidan. Aktinobakteri
endofit juga dapat menghasilkan senyawa bioaktif. Penelitian ini bertujuan untuk
mengetahui keberadaan aktinobakteri endofit X. granatum dan mengetahui aktivitas
antioksidannya. Metode penelitian diawali dengan isolasi aktinobakteri endofit dari
buah, biji, dan daun menggunakan media selektif Humic acid (HV). Selanjutnya
dilakukan karakterisasi isolat yang diperoleh menggunakan empat media berbeda
yaitu YSA, ISP2, ISP3, dan ISP4.
Aktivitas antioksidan dari supernatant aktinobakteri dan ekstrak etil asetat
aktinobakteri dilakukan menggunakan metode DPPH. Hasil penelitian
menunjukkan bahwa dari X. granatum diperoleh 15 isolat aktinobakteri endofit dari
buah, biji, dan daun. Isolat aktinobakteri endofit X. granatum menunjukkan
perbedaan warna pada miselium aeria dan miselium substrat, serta perbedaan
penataan rantai spora. Supernatan XGF11, XGF12, dan XGF4 menghasilkan aktivitas
antioksidan tertinggi yaitu: 57.30%, 49.77%, dan 41.90% penghambatan. Kapasitas
antioksidan ekstrak etil asetat XGF12 adalah 501.60 μg AEAC/mg ekstrak, 465.47
μg AEAC/mg ekstrak untuk ekstrak etil asetat XGF11, dan 455.53 μg AEAC/mg
ekstrak untuk ekstrak etil asetat XGF4. Ekstraksi DNA aktinobakteri endofit
dilakukan berdasarkan Presto Mini gDNA Bacteria Kit. Berdasarkan hasil analisis
gen 16S rRNA, isolat XGF11 dan XGF12 menunjukkan kemiripan masing-masing
99.42% dan 99.16% dengan Streptomyces xylanilyticus SR2-123 dan XGF4
memiliki kemiripan 99.82% dengan Brevibacterium sediminis FXJ8.269. Xylocarpus granatum produces various bioactive compounds with diverse biological activities, one of them as an antioxidant. Endophytic actinobacteria can also produce bioactive compounds. This study aimed to explore the existence of the endophytic actinobacteria of X. granatum and determine its antioxidant activity. The research method begins with the isolation of endophytic actinobacteria from fruits, seeds, and leaves using selective media Humic Acid (HV). Furthermore, characterizing the obtained isolates using four different media, namely YSA, ISP2, ISP3, and ISP4. Measured antioxidant activity for actinobacterial supernatants and actinobacterial ethyl acetate extracts using the DPPH method. The results showed that from X. granatum, 15 isolates of endophytic actinobacteria were obtained from fruit, seeds, and leaves. Endophytic actinobacteria isolates from X. granatum showed different colors of aerial mycelium, substrate mycelium, and spore chain type. XGF11, XGF12, and XGF4 isolates produced high antioxidant activity of the supernatant; 57.30%, 49.77%, and 41.90% inhibition, respectively. The antioxidant capacity of XGF12 ethyl acetate extract was 501.60 μg AEAC/mg extract and 465.47 μg AEAC/mg extract for XGF11 ethyl acetate extract. DNA extraction and 16S rRNA amplification from endophytic actinobacteria X. granatum according to Presto Mini gDNA Bacteria Kit Protocol. Based on 16S rDNA gene analysis, isolates XGF12 and XGF11 showed 99.16% and 99.42% similarities with Streptomyces xylanilyticus, respectively. Meanwhile, XGF4 showed to have 99.82% similarities with Brevibacterium sediminis. |
URI: | http://repository.ipb.ac.id/handle/123456789/110042 |
Appears in Collections: | MT - Mathematics and Natural Science |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Watermark_Halaman Awal - TESIS - (G351180271) MARINA RATTE.pdf Restricted Access | Cover | 453.72 kB | Adobe PDF | View/Open |
Watermark_Fullteks - TESIS - (G351180271) MARINA RATTE.pdf Restricted Access | Fullteks | 1.02 MB | Adobe PDF | View/Open |
Watermark_Lampiran - TESIS - (G351180271) MARINA RATTE.pdf Restricted Access | Lampiran | 1 MB | Adobe PDF | View/Open |
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