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Title: | Isolasi, Identifikasi, dan Formulasi Media Bakteri Endofit untuk Membatasi Serangan Cendawan Ganoderma pada Kelapa Sawit |
Other Titles: | Isolation, Identification, and Media Formulation of Endophytic Bacteria to Limit Ganoderma Fungus Attacks on Oil Palm |
Authors: | Rahayuningsih, Mulyorini Rizal, Syaiful |
Issue Date: | 2021 |
Publisher: | IPB University |
Abstract: | Ganoderma boniense merupakan cendawan patogen yang menyebabkan
penyakit busuk pangkal batang (BPB) pada tanaman kelapa sawit. Penelitian ini
mengkaji suatu alternatif dalam mengendalikan G. boninense melalui pemanfaatan
agens pengendali hayati (APH) yang lebih ramah lingkungan daripada penggunaan
fungisida komersial. Seleksi bakteri endofit sebagai APH dilakukan melalui isolasi
bakteri dari akar kelapa sawit, mengukur persen zona hambatan terhadap isolat G.
boninense secara in vitro, dan uji hipersensitif pada tanaman tembakau. Isolat
bakteri endofit terpilih A2 dan C5 memiliki daya hambat terhadap G. boninense
berturut-turut sebesar 35% dan 47%. Kedua isolat tersebut teridentifikasi secara
molekuler berturut-turut sebagai bakteri Lysinibacillus xylanilyticus (99,85%) dan
bakteri Aeromonas hydrophila (99,64%). Bakteri endofit terpilih diformulasikan ke
dalam formulasi kompos, tepung, dan cair. Ketiga jenis formula dapat
mempertahankan viabilitas sel sampai dengan 106 cfu/ml serta lebih stabil dan
adaptif pada formulasi cair dengan kepadatan berkisar (8,0–9,0)×106
cfu/ml pada
akhir minggu pengamatan dibandingkan formulasi lainnya. Ganoderma boninense is a pathogenic fungus that causes basal stem rot (BSR) diseases in oil palm plantations. This research examined an alternative in controlling G. boninense through the use of biological control agents (BCAs) which was more environmentally friendly than the use of commercial fungicide. Endophytic bacteria as BCAs were selected by isolating bacteria from oil palm roots, measuring the percent zone of inhibition against G. boninense based on in vitro test, and presenting hypersensitivity test on tobacco plant. Selected endophytic bacterias namely A2 and C5 had inhibiton against G. boninense of 35% and 47%, respectively. Those isolates were identified molecularly as Lysinibacillus xylanilyticus (99,85%) and Aeromonas hydrophila (99,64%), respectively. The selected endophytic bacterias were formulated in compost, flour, and liquid formulation. Those three types of formula could maintain cell viability up to 106 cfu/ml and are more stable also adaptive in liquid formulation within a range of (8,0–9,0)×106 cfu/ml at the end of the observation compared to other formulations. |
URI: | http://repository.ipb.ac.id/handle/123456789/109415 |
Appears in Collections: | UT - Agroindustrial Technology |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cover_F34170084_Syaiful Rizal.pdf Restricted Access | Cover | 937.37 kB | Adobe PDF | View/Open |
F34170084_Syaiful Rizal.pdf Restricted Access | Full text | 3.08 MB | Adobe PDF | View/Open |
Lampiran_F34170084_Syaiful Rizal.pdf Restricted Access | Lampiran | 2.26 MB | Adobe PDF | View/Open |
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