Please use this identifier to cite or link to this item: http://repository.ipb.ac.id/handle/123456789/109275
Title: Identifikasi Biometrik Telur Cacing Fasciola gigantica menggunakan Dino Lite dan Image-J
Other Titles: Biometric Identification of Fasciola gigantica Worm Eggs using Dino Lite and Image-J
Authors: Arif, Ridi
Novelina, Savitri
Selviana, Rini
Issue Date: 2021
Publisher: IPB University
Abstract: Fasciolosis di Indonesia merupakan masalah penting pada ruminansia yang disebabkan oleh infeksi Fasciola gigantica. Kejadian fasciolosis dapat diidentifikasi dengan melakukan pemeriksaan feses untuk menemukan telur cacingnya. Penelitian ini bertujuan mendeskripsikan telur Fasciola gigantica berdasarkan panjang, lebar, radius mayor, radius minor, luas, keliling, eksentrisitas, dan panjang latus rectum telur. Sebanyak tiga sampel awetan cacing digerus dengan menggunakan mortar dan stemper kemudian diletakkan diatas cover glass. Preparat diletakkan dibawah mikroskop yang sudah terhubung dengan microscope eye-piece camera (Dino Eye AM4023X) dengan perbesaran objektif 10×, 20×, dan 40×. Masing-masing sampel preparat diambil 10 gambaran telur cacing. Perangkat lunak Image-J dan Dino Lite membantu proses pengidentifikasian biometrik telur cacing ini. Hasil penelitian menunjukkan biometrik cacing tersebut memiliki rata-rata panjang telur 161,28 ± 8,53 μm, lebar telur 92,40 ± 5,40 μm, radius mayor telur 80,64 ± 4,27 μm, radius minor telur 46,29 ± 2,68 μm, luas telur menggunakan rumus 35256,05 ± 2322,64 μm2, luas telur menggunakan Image-J 29495,11 ± 1980,09 μm2, keliling telur menggunakan rumus 487,31 ± 18,94 μm, keliling telur menggunakan Image-J 210,02 ± 5,64 μm, eksentrisitas telur 0,81 ± 0,04 e, dan panjang latus rectum telur 88,99 ± 13,95 μm. Telur Fasciola gigantica memiliki bentuk yang sama pada kedua kutub, operkulum pada salah satu kutubnya, kerabang telur tipis, dan blastomer yang memenuhi rongga telur.
Fasciolosis in Indonesia is an important problem in ruminants caused by infection with Fasciola gigantica. The incidence of fasciolosis can be identified by examining the feces to find the worm eggs. This research aims to describe Fasciola gigantica eggs based on length, width, radius major, radius minor, area, circumference, eccentricity, and length of the latus rectum of the egg. A total of 3 samples of preserved worms were ground using a mortar and stemper and then placed on a cover glass. The preparations were placed under a microscope that was connected to a microscope eye-piece camera (Dino Eye AM4023X) with 10×, 20×, and 40× magnifications. For each sample preparation, 10 images of worm eggs were taken. Image-J and Dino Lite software assist in the biometric identification of these worms. The results showed that the biometrics of these worms had an average egg length of 161,28 ± 8,53 m, egg width 92,40 ± 5,40 m, egg major radius 80,64 ± 4,27 m, egg minor radius 46,29 ± 2,68 m, egg area using the formula 35256,05 ± 2322,64 m2, egg area using Image-J 29495,11 ± 1980,09 m2, egg circumference using the formula 487,31 ± 18,94 m, egg circumference using Image-J 210,02 ± 5,64 m, egg eccentricity 0,81 ± 0,04 e, and egg latus rectum length 88,99 ± 13,95 m. Fasciola gigantica eggs had equal shape at both poles, operculum at one pole, thin egg shell, and blastomere that fills the egg cavity.
URI: http://repository.ipb.ac.id/handle/123456789/109275
Appears in Collections:UT - Animal Disease and Veterinary Health

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