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DC Field | Value | Language |
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dc.contributor.advisor | Sukarno, Nampiah | - |
dc.contributor.advisor | Batubara, Irmanida | - |
dc.contributor.author | Khairillah, Yuyun Nisaul | - |
dc.date.accessioned | 2021-08-10T03:18:28Z | - |
dc.date.available | 2021-08-10T03:18:28Z | - |
dc.date.issued | 2021-08 | - |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/108286 | - |
dc.description.abstract | Tanaman kopi merupakan tanaman tropis yang menjadi komoditas unggulan dalam sub sektor perkebunan di Indonesia. Keberhasilan pertumbuhan dan produksi tanaman kopi ialah adanya interaksinya dengan cendawan endofit yang tumbuh pada semua jaringan tanaman dan cendawan saprob yang tumbuh pada serasah dan rizosfir tanaman. Cendawan endofit dan saprob dilaporkan menghasilkan berbagai senyawa metabolit sekunder yang diantaranya bersifat antimikrob yang menekan pertumbuhan cendawan patogen Candida albicans, penyebab penyakit kandidiasis pada manusia. Penelitian ini bertujuan untuk mempelajari keanekaragaman cendawan endofit dan saprob pada tanaman kopi robusta (C.canephora), menganalisis potensinya sebagai anti C.albicans, serta menentukan dugaan senyawa aktif yang dapat mengendalikan cendawan patogen tersebut. Sebanyak 30 isolat cendawan yang memiliki karakter morfologi koloni berbeda yaitu tiga isolat dari daun, sembilan isolat dari serasah dan 18 isolat dari rizosfer. Warna koloni yang teramati bagian atas yaitu merah muda, putih, putih keabu-abuan, putih kekuningan, putih kehijauan, hijau muda dan hijau zaitun. Tekstur permukaan koloni beragam yaitu bertekstur halus, menggulung, seperti kapas, dan bergranulla. Hifa cendawan septat dan hanya membentuk spora aseksual. Kecepatan pertumbuhan koloni cepat sampai lambat. Hasil identifikasi berdasarkan karakter morfologi menunjukkan di peroleh tujuh genus yaitu Fusarium, Humicola, Neocosmopora, Rigidosporus, Talaromyces, Tricoderma, dan Xylaria. Sebanyak 17 isolat mempunyai aktivitas anti C.albicans dengan kisaran zona bening antara 2.50 mm – 15.53 mm, dua isolat tergolong dalam penghambat kuat, sembilan isolat tergolong sedang, dan enam tergolong lemah. Penghambatan tertinggi di peroleh dari cendawan Trichoderma TN- 103-1 dari rizosfer. Hasil identifikasi molekuler, 17 isolat positif anti Candida terdiri dari 11 spesies yaitu F.equiseti, F. graminearum, F. oxysporum. F. sinensis, F. solani, H. fuscoatra, R. microporus, T. macrosporus, T. hamatum, T. ovalisporum dan X. curta. Filtrat kultur cendawan dengan anti C.albicans terbaik yaitu T. hamatum TN-103-1 memiliki nilai penghambatan mirip dengan nilai penghambatan pada uji kultur yaitu sebesar 14.40 mm. Hal ini menunjukkan komponen aktif kultur terdapat pada filtrat (ekstraseluler). Filtrat diekstraksi menggunakan pelarut etil asetat dan n-heksana. Ekstrak etil asetat menghasilkan rendemen lebih kecil yaitu sebesar 0.80% dibandingkan ekstrak n-heksana (0.94%). Ekstrak etil asetat T.hamatum TN- 103-1 memiliki nilai konsentrasi hambat minimum 0.5% yang lebih aktif dibandingkan dengan ekstrak n-heksana dengan konsentrasi hambat minimun sebesar 15%, bahkan lebih baik dibandingkan kontrol positif menggunakan nistatin (100.000 U/ml) dengan konsentrasi hambat minimum yang lebih besar yaitu 1.0%. Dugaan senyawa aktif pada ekstrak etil asetat adalah 9-octadecenoic acid. | id |
dc.description.abstract | Coffee is a tropical plantation plant that is an essential commodity in Indonesia. The growth and production of the coffee plant are a result of interaction between the coffee plant and endophytic fungi that grow in all plant tissues and saprobes on plant litter and in the rhizosphere. Endophytic and saprophytic fungi are reported to produce various secondary metabolites, including antimicrobials that suppress the growth of the fungal pathogen Candida albicans, the cause of candidiasis in humans. This study aims to study the diversity of endophytic fungi and saprobes on robusta coffee plants (C. canephora), analyze their potential as anti-C. albicans, and determine the suspected active compounds that can control these fungal pathogens. The total of 30 fungal isolates that had different colony morphology characters were obtained. Three isolates were isolated from the leaves, nine isolates from the litter, and 18 isolates from the rhizosphere. The colors of the colonies observed were pink, white, greyish-white, yellowish-white, greenish-white, light green, and olive green. The surface texture of the colonies varied, namely smooth, rolled, like cotton, and granular. The fungal hyphae are septate and only form asexual spores. The colony growth rate is fast to slow. Based on morphological characters, the fungi identified to seven genera, namely Fusarium, Humicola, Neocosmopora, Rigidosporus, Talaromyces, Trichoderma, and Xylaria. A total of 17 isolates had anti-C. albicans activity with a clear zone range from 2.50 mm to 15.53 mm. Among them, two isolates were classified as strong, nine isolates were moderate, and six were weak inhibitors. The highest inhibition was obtained from the fungus Trichoderma TN-103-1 from the rhizosphere. The results of molecular identification showed that 17 positive isolates belong to 11 species, namely F. equiseti, F. graminearum, F. oxysporum. F. sinensis, F. solani, H. fuscoatra, R. microporus, T. macrosporus, T.hamatum, T. ovalisporum and X. curta. Fungal culture filtrate of T. hamatum TN-103-1 had the best anti-C. albicans, with an inhibitory value of 14.40 mm. This is similar to that of the inhibition value in the culture test. This shows that the active components of the culture are present in the fungal filtrate (extracellular). The filtrate was extracted using ethyl acetate and n-hexane as solvent. The ethyl acetate extract produced a smaller yield of 0.80% than the n-hexane extract (0.94%). The ethyl acetate extract of T.hamatum TN-103-1 had a minimum inhibitory concentration of 0.5%, which was more active than the n-hexane extract with a minimum inhibitory concentration of 15%, even better than the positive control nystatin (100,000 U/ml) with greater minimum inhibitory concentration, namely 1.0%. The suspected active compound in the ethyl acetate extract was 9-octadecenoic acid. | id |
dc.language.iso | id | id |
dc.publisher | IPB-University | id |
dc.subject.ddc | Cendawan Endofit dan Cendawan Saprob | id |
dc.title | Keanekaragaman, potensi sebagai anti Candida albicans dan senyawa aktif cendawan pada tanaman kopi robusta (Coffea canephora Pierre Ex A. Froenher) | id |
dc.title.alternative | Diversity, anti Candida albicans potency and bioactive compound of Coffea canephora associated fungi | id |
dc.type | Thesis | id |
dc.subject.keyword | cendawan endofit | id |
dc.subject.keyword | cendawan saprob | id |
dc.subject.keyword | filtrat ekstrak | id |
dc.subject.keyword | ITS rDNA | id |
dc.subject.keyword | zona bening | id |
Appears in Collections: | MT - Mathematics and Natural Science |
Files in This Item:
File | Description | Size | Format | |
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Cover.pdf | Cover | 2 MB | Adobe PDF | View/Open |
Yuyun Nisaul Khairillah-G351180011-Full Text.pdf Restricted Access | Fullteks | 5.18 MB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 4.15 MB | Adobe PDF | View/Open |
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