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Title: | Penambatan Molekuler Komponen Kimia Limbah Palm Oil Mill Effluent dengan Beberapa Enzim Mikrobial sebagai Agen Bioremediasi Potensial |
Other Titles: | Molecular Docking of Potential Microbial Enzyme as Palm Oil Mill Effluent Bioremediation Agents |
Authors: | Ambarsari, Laksmi Andrianto, Dimas Zahra, Hafizh |
Issue Date: | 2021 |
Publisher: | IPB University |
Abstract: | Palm oil mill effluent (POME) yang berasal dari industri kelapa sawit
merupakan salah satu penyebab utama pencemaran air karena kaya akan bahan
organik dan warnanya yang gelap. Limbah POME memiliki beberapa zat penyebab
sifat uniknya, salah satunya adalah melanoidin. Tidak hanya melanoidin, komponen
fenolik yang terdapat pada POME juga diketahui bersifat fitotoksik bagi lingkungan
akuatik. Beberapa enzim mikroba potensial sudah dianggap mampu mendegradasi
bahan kimia ini, tetapi mekanismenya tidak diketahui. Melalui penelitian ini, enzim
potensial tersebut (α-glukosidase, β-glukosidase, β-mannanase, lakase, dan mangan
katalase) digunakan untuk memvisualisasikan pengikatan kompleks reseptor-ligan
dalam mencari agen bioremediasi POME yang sesuai. Analisis penelitian dilakukan
dengan Autodock Vina dan skor penambatan mewakili kekuatan afinitas. Hasil
penelitian menunjukkan bahwa melanoidin yang berkontribusi terhadap warna
gelap POME memiliki nilai docking terbaik yaitu -10,1 kkal/mol dengan mangan
katalase. Hasil akhir menunjukkan bahwa semua reseptor memiliki afinitas tinggi
terhadap melanoidin, yang menunjukkan bahwa enzim ini melakukan reaksi
spontan terhadap komponen kimia limbah POME Palm oil mill effluent (POME) originated from palm oil industries is one of the significant causes of water pollution because of its richness in organic matters and dark color. It has been discovered that POME has several substances that cause its distinct characteristics, namely melanoidin. Several phenolic components found in POME are also known to be phytotoxic for the aquatic environment. Certain potential microbial enzymes are already presumed capable of degrading these chemicals, but the exact mechanism is unknown. Here I employed molecular docking of these possible enzymes (i.e., α-glucosidase, β-glucosidase, β-mannanase, laccase, and manganese catalase) to visualize the ligand-receptor complex binding in searching for POME potential bioremediation agent. The result analysis uses the Autodock Tool v1.5.6, and the docking score represents the affinities of the bond strength. The results show that melanoidin has the lowest docking score with -10.1 kcal/mol into manganese catalase. The results demonstrate that all of the receptors possess a high affinity towards melanoidin, indicating that these enzymes perform spontaneous reactions towards POME waste chemical components |
URI: | http://repository.ipb.ac.id/handle/123456789/107950 |
Appears in Collections: | UT - Biochemistry |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cover, Lembar Pernyataan, Abstrak, Lembar Pengesahan, Prakata dan Daftar Isi.pdf Restricted Access | Cover | 5.93 MB | Adobe PDF | View/Open |
G84170084_Hafizh Zahra.pdf Restricted Access | Fullteks | 5.93 MB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 5.93 MB | Adobe PDF | View/Open |
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