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Title: | Potensi Nanoenkapsulasi Ekstrak Daun Singkong Sebagai Imunomodulator pada Burung Puyuh yang Diberi Metilprednisolon |
Other Titles: | Potential Nanoencapsulation Extracts of Cassava Leaf as Immunomodulators in Quails given Methylprednisolone |
Authors: | Santoso, Koekoeh Setiyaningsih, Surachmi Nurmustari, Fitria |
Issue Date: | 26-Jul-2021 |
Publisher: | IPB University |
Abstract: | Burung puyuh mudah mengalami stres sehingga memicu penurunan fungsi
sistem kekebalan tubuh. Daun singkong dapat meningkatkan sistem kekebalan
tubuh. Penelitian ini bertujuan untuk mengetahui potensi ekstrak daun singkong dan
penggunaan teknologi nanoenkapsulasi untuk memperbaiki sistem imun pada
puyuh yang mengalami stres oksidatif akibat pemberian metilprednisolon. Ekstrak
daun singkong dibuat menggunakan metode maserasi, nanoenkapsulasi dibuat
menggunakan metode gelasi ionik dengan penyalut kitosan. Penelitian ini terdiri
dari 6 kelompok dan masing-masing kelompok terdapat 4 ekor burung puyuh.
Setiap kelompok diberikan metilprednisolon dosis 0,70 mg/Kg BB, antigen sel
darah merah domba dan vaksin aktif newcastle desease (ND) La Sota. Perlakuan
yang diberikan KN (Kontrol negatif: tanpa perlakuan), KP (Kontrol positif:
Stimuno 0.75 mg/kgBB), N1 (Nanoenkapsulasi 19.0512 mg/KgBB), N2
(Nanoenkapsulasi 21.168 /kgBB), N1T (Nanoenkapsulasi 19.0512/kgBB dengan
tween 0.3%) dan N2T (Nanoenkapsulasi 21.168 mg/KgBB dengan tween 3%).
Hasil penelitian menunjukkan bahwa pemberian nanoenkapsulasi ekstrak daun
singkong dengan atau tanpa penambahan tween 80 berpengaruh signifikan (P<0.05)
terhadap peningkatan total leukosit dan limfosit, menurunkan heterofil dan rasio
heterofil-limfosit serta memacu terbentuknya antibodi terhadap SDMD maupun
titer antibodi protektif terhadap ND. Hal ini mengindikasikan bahwa
nanoenkapsulasi ekstrak daun singkong memiliki potensi yang baik sebagai
imunomodulator. Quails are easily stressed, triggering a decrease in immune system function. Cassava leaves can improve the immune system. The research aims to study the potency of cassava leaves nanoencapsulation as immunomodulators for stressed quails due to methylprednisolone. Cassava leaves extract were prepared using maceration method, nanoencapsulation were using ionic gelation method with chitosan coating. This study consisted of 6 groups each group contained 4 quails. Each group was given methylprednisolone at a dose of 0.70 mg/Kg BW, sheep red blood cell (SRBC) antygen, and Newcastle Desease (ND) La Sota active vaccine. Treatments given were KN (Negative control: without treatment), KP (Positive control: Stimuno 0.75 mg/Kg BW), N1 (Nanoencapsulation 19.0512 mg/Kg BW), N2 (Nanoencapsulation 21.168 mg/Kg BW), N1T (Nanoencapsulation 19.0512 mg/Kg BW with 0.3% tween 80) and N2T (Nanoencapsulation 21.168 mg/Kg BW with 3% tween 80). The results showed that cassava leaf extract nanoencapsulation with or without tween 80 had a significant effect (P<0.05) on the increase leukocytes number and lymphocytes, decreased of heterophil and ratio of heterophils to lymphocytes, produced SRBC antibody titers and protective antibody titer to ND. This finding indicates that cassava leaf extract nanoencapsulation has good potency as an immunomodulator. |
URI: | http://repository.ipb.ac.id/handle/123456789/107809 |
Appears in Collections: | UT - Anatomy, Phisiology and Pharmacology |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cover.pdf | Cover | 418.83 kB | Adobe PDF | View/Open |
Fitria Nurmustari_B04170075.pdf Restricted Access | Fullteks | 9.27 MB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 839.86 kB | Adobe PDF | View/Open |
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