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http://repository.ipb.ac.id/handle/123456789/107450
Title: | Studi Tentang Aktivitas Antioksidasi dan Antimikroba Ekstrak Air Liur Lintah Lokal Indonesia (Hirudinaria manillensis). |
Authors: | Astuti, Dewi Apri Jayanegara, Anuraga Rahminiwati, Min Malik, Burhanudin |
Issue Date: | 2021 |
Publisher: | IPB University |
Abstract: | Pengujian aktivitas antioksidasi dan antimikroba ekstrak air liur lintah lokal
Indonesia dilakukan. Lintah diperoleh dari Situgede Bogor, Sawangan Depok, dan
Kabupaten Sijunjung, Sumatera Barat. Penelitian dilakukan dalam rancangan acak
lengkap dengan tiga perlakuan dan lima ulangan. Perlakuan terdiri atas tiga daerah asal
lintah. Pengukuran dilakukan terhadap jumlah, kandungan protein total, nilai IC50, zona
penghambatan pertumbuhan bakteria, dan konsentrasi penghambatan minimum dari
ekstrak kasar air liur lintah (EKALL). Hasil penelitian menunjukkan bahwa jumlah
EKALL yang diperoleh adalah 3,696-4,633 g/g BB dan kandungan protein total EKALL
adalah 48,303-64,742 μg/g. Nilai IC50 adalah 47-108 μg/g pada EKALL dan 3,312 μg/g
pada asam askorbat. Aktivitas antimikroba ditemukan pada pertumbuhan S. aureus
dengan konsentrasi penghambatan minimum sebesar 2,472-4,528 μg/ml, tetapi tidak
ditemukan pada pertumbuhan E. Coli. Antioxidative and antimicrobial activities of saliva extract of Indonesian leeches were assessed. Leeches were obtained from Situgede Bogor, Sawangan Depok, and Sijunjung Regency, West Sumatra. A completely randomized design with three treatments and five replicates was used. Treatments consisted of three places of leech origins. Measurements were taken on the amount, total protein content, IC50 value, zone of inhibition to bacterial growth, and minimum inhibitory concentration of crude leech saliva extract (CLSE). Results showed that the amount of CLSE obtained was 3.696- 4.633 g/g BW and total protein content of CLSE was 48.303-64.742 μg/g. The IC50 value was 47-108 μg/g while that of ascorbic acid was 3.312 μg/g. Antimicrobial activity was found to the growth of S. aureus but not to the growth of E. coli with a minimum inhibitory concentration at 2.472-4.528 μg/ml. |
URI: | http://repository.ipb.ac.id/handle/123456789/107450 |
Appears in Collections: | DT - Animal Science |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cover.pdf Restricted Access | Cover | 370.18 kB | Adobe PDF | View/Open |
D261154011_Burhanudin Malik.pdf Restricted Access | Fullteks | 10.08 MB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 183.32 kB | Adobe PDF | View/Open |
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