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http://repository.ipb.ac.id/handle/123456789/105461
Title: | Efek Ekstrak Daun Cengkeh (Syzygium aromaticum L.) terhadap Bakteri Saluran Pencernaan dan Neurogenesis di Hippocampus pada Mencit (Mus musculus). |
Other Titles: | The Effect of Clove Leaves Extract (Syzygium aromaticum L.) on Digestive Bacteria and Neurogenesis in Hippocampus on Mice (Mus musculus) |
Authors: | Astuti, Rika Indri Juliandi, Berry Saadah, Lailatul |
Issue Date: | 2020 |
Publisher: | IPB University |
Abstract: | Neurogenesis adalah proses lahirnya neuron baru pada dentate gyrus di
hipokampus. Mikrobiota saluran pencernaan dapat mempengaruhi neurogenesis
melalui sistem komunikasi dua arah, yang terjadi antara otak dan saluran
pencernaan (gut-brain axis). Cengkeh yang kaya akan polifenol dapat
mempengaruhi neurogenesis. Penelitian ini bertujuan mengetahui pengaruh
pemberian ekstrak daun cengkeh yang memiliki kandungan senyawa polifenol
terhadap neurogenesis yang mempengaruhi kinerja memori dan tingkat kecemasan
pada mencit dan bagaimana hubungannya dengan komposisi bakteri pada saluran
pencernaan. Dalam penelitian ini, metode light-dark transition test dan y-maze
alteration test digunakan untuk analisis perilaku. Mikrobiota feses mencit diisolasi
dengan metode media selektif. Perbandingan jumlah koloni mikrobiota dihitung
melalui metode total plate count. Dosis tinggi P400 memiliki kinerja memori
spasial paling rendah setelah 4 minggu pemberian perlakuan (M4). Dosis tinggi
P400 pada M4 juga menunjukkan tingkat kecemasan paling tinggi yang dialami
oleh mencit. Sebaliknya pada kelompok perlakuan P100 dan P200 mencit
menunjukkan tingkat kecemasan yang lebih rendah pada M4. Hasil analisis
menunjukkan semakin rendah dosis yang diberikan tingkat neurogenesisnya juga
semakin baik (NPC terbanyak P100). Kuantifikasi mikrobiota P400 meningkatkan
konsentrasi bakteri Escherichia coli namun, menurunkan konsentrasi BAL. Hasil
uji perilaku memperlihatkan tingkat dosis yang diberikan berkaitan dengan lama
waktu pemberian perlakuan. Neurogenesis is the process of producing new neurons at the dentate gyrus in the hippocampus. The digestive tract microbiota can influence neurogenesis through the two-way communication system between the brain and the digestive tract (gut-brain axis). Cloves are rich in polyphenols that can affect neurogenesis. This study aims to determine the effect of giving clove leaf extract which contains polyphenol compounds on neurogenesis which affects memory performance and anxiety levels in mice and how it relates to the composition of bacteria in the digestive tract. Behavioral analysis was carried out using light-dark transition test and y-maze alternation test. The isolation of the fecal microbiota a was done using selective media method. The number of microbiota colonies was calculated using the total plate count method. The high dose of P400 has the lowest spatial memory performance following four weeks of treatment (M4). The high dose of P400 on M4 also showed the highest level of anxiety experienced by the mice. Whereas, in the treatment group P100 and P200 mice showed the lowest anxiety level at M4. Our data indicate that the lower of the dose given, the better of neurogenesis rate level (most NPC was P100). P400 treatment increased the concentration of Escherichia coli bacteria yet decreased the concentration of BAL. Behavioral test results shows that the dose level given is related to length of giving treatment time. |
URI: | http://repository.ipb.ac.id/handle/123456789/105461 |
Appears in Collections: | UT - Biology |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cover, Lembar Pengesahan, Prakata, Daftar Isi.pdf Restricted Access | Cover | 2.48 MB | Adobe PDF | View/Open |
G34160018_Lailatul Saadah.pdf Restricted Access | Full Text | 2.81 MB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 2.31 MB | Adobe PDF | View/Open |
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