Bioremediasi Tanah Tercemar Diazinon Secara Ex Situ dengan Menggunakan Kompos Limbah Media Jamur (Spent Mushroom Compost)
Abstract
Pesticide is a xenobiotic compound and rexalcytran which should be handled seriously in order to prevent human and environment from those negative effects. One of the methods which can be utilized is bioremediation technique which is utilizing fresh spent mushroom compost (SMC). The diazinon contaminated soil whith the concentration of 500 ppm, 1000 ppm and1500 ppm, was added with SMC of 10%, 20% and 30%, then incubated for 28 days. The reduction of diazinon concentrate was analyzed every week through extracted sample with etyl acetat and measured by spectrophotometer. The data processing was conducted by using respon surface method (RSM). Based on this analysis, the maximum point of diazinon concentrate reduction was 88% with the treatment of combination of 30% compost and 1000 ppm diazinon, it was obatined on the 14th. On the 21st day the diazinon concentration reduction was 91.8%. It was the result from combination of 25% compost and 1000 ppm diazinon. On 28th day, the diazinon concentration reduction was 97.5%, result from the treatment of 26% compost and 1000 ppm diazinon. The diazinon degradation process can be effectively ferformed by adding 15-30% compost, with 1000 ppm of diazinon within 21 days incubation. Some of bacteria have been isolated from SMC. that were, Bacillus mycoides, Bacillus cereus, Bacillus brevis, Pseudomonas stutzeri, dan Chromobacterium spp. Those bacteria were able to growth in solid medium which contains diazinon up to 500 ppm, except Bacillus brevis. The ability of bacteria to degrade diazinon based on their properties is forming a clear zone around the colony which is growth in solid medium of mineral salt peptone yeast (MSPY). Apparently, it is only Bacillus cereus is able to degrade diazinon up to 1700 ppm.
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- MT - Agriculture Technology [2276]