Aplikasi Enzim Bakteri Selulotik dan Xilanolitik Dalam Dekomposisi Substrat Limbah Tanaman Padi

Abstract

The cellulolytic and xilanolytic bacteria are among organisms that is important to decompose organic matters from agriculture with higher lingo-cellulose compound such as rice straw. This research aims to isolate cellulolytic bacteria from agricultural soil in West and Central Java, and to study the cellulolytic and xilanolytic capacity of selected bacteria (45I-3 and 234P-16) to decompose rice straw. Method of this research comprised isolation and selection of cellulolytic bacteria which have a higher cellulolytic activity and was able to grow under higher temperature (50 oC). The selected bacteria than were analysed their ability to decompose rice straw for 6 weeks. Four combinations of bacteria including C4-4 + Xilanolytic (A), C5-1 + Xilanolytic (B), C11-1 + Xilanolytic (C), 45I-3 + 234P-16 (D), and control (without bacteria) were applied as inoculant of rice straw decomposition. The parameters analysed including the changes of pH-H2O, temperature, C-organic, total N and NNH4 +, decomposition rate, remaining weight, macro-micro nutrient content as well as physical properties of the substrate. There were 3 prospective isolates of cellulolytic bacteria resulted from the total of 31 isolates we found, i.e. C4-4, C5-1, and C11-1. The pH-H2O value of C4-4 + Xilanolytic (A) and C5-1 + Xilanolytic (B) was relatively more stable. C/N ratio of all treatments decreased after 3 weeks incubation. The C/N ratio value of A, B, C, D and E treatments were 22.484, 23.425, 27.494, 26.823, and 29.525 respectively. Decompositon rate all of combination A, B, C, D treatments were quicker than the control. The content of macro-micro nutrient of A, B, C, D treatments increased in the end of measuremant, while the control didn’t. The physical characteristic of substrate including the shape and colour was better in A and B treatments that others. The result indicated that the combination of C4-4 + Xilanolytic (A) and C5-1 + Xilanolytic (B) bacteria was the best combination of cellulolytic and xilanolytic bacteria for decomposition of rice straw.