Pemakaian Medium CR1aa untuk Produksi Embrio Kambing In Vitro dan Kriopreservasi dengan Metode Vitrifikasi
The Use of Crlaa Media For in Vitro Production of Goat Embryos and Cryopreservation by Vitrification Methods
Abstract
Pemakaian medium CR1aa sebagai medium alternatif dalam proses fertilisasi in vitro (FIV) pada ternak kambing yang meliputi pematangan dan fertilisasi posit dan kultur embrio in vitro. Bertujuan untuk menyederhanakan dan mengefisiensikan medium yang dpat digunakan dalam memproduksi embrio kambing secara in vitro. The objective of this study was to examine the use of CRlaa media for supporting in vitro maturation, fertilization and early embryo development of goat. In experiment 1. Oocytes were matured in TCM 199 or CRlaa medium for 18, 22, 26 and 30 h at 38.5°C in 5% CO2 in air. Three different sperm treatments/IVF media were used: BO, TALP or CRlaa medium.
