Tingkat Kejadian Escherichia coli Penghasil Extended Spectrum β-Lactamase di Feses Sapi di Rumah Potong Hewan Ruminansia Kota Bogor
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Date
2015Author
Sukmawinata, Eddy
Lukman, Denny Widaya
Latif, Hadri
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Escherichia coli belongs to the family of Enterobacteriaceae and is common in the gastrointestinal microflora in human and animals. This bacteria has capability to get and disseminate the resistant genes for antibiotics. One of the currently most important resistance mechanisms in E. coli is production of the extended spectrum β-lactamases (ESBL). Extended spectrum β-lactamases producing E. coli confers resistance to the majority of the commonly used β-lactam antimicrobials, including third generation cephalosporins. The slaughterhouse may be one of the major ESBL reservoirs in food animals. This study was aimed to determine the occurrence of ESBL producing E. coli from cattle feces in Bogor Slaughterhouse. This study was conducted using a cross sectional study. The sample size was calculated based on 95% of convidence level, 9.13% of margin of error, and 50% predicted prevalence and resulted a total of 120 samples for the study. The study was performed by isolation and identification of ESBL producing E. coli. Confirmation test for presence of ESBL was performed by disc diffusion test based on recomendation from Clinical and Laboratory Standards Institute (2014). The results showed that 19 isolates of E. coli were positive producing ESBL (15.8%). CTX-M ESBL was presumed more dominant than others because all of ESBL isolates showed positive results when tested by cefotaxim and cefotaxim combining with clavulanic acid. Extended spectrum β-lactamase producing E. coli in feceses could contaminate the environment and foods. Resistance of ESBL producing E. coli to antibiotics can be transmitted to other bacteria by plasmid. The presence of ESBLs causes limited therapeutic options for bacterial infection. Escherichia coli strains can also cause a wide variety of intestinal and extra-intestinal diseases, such as diarrhea, urinary tract infections (UTI), septicemia, and neonatal meningitis.
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- MT - Veterinary Science [909]