Pengaruh Kurkumin terhadap Skleroderma dan Fibrosis Paru-paru Akibat Aplikasi Bleomisin pada Mencit (Mus musculus)

Abstract

Curcumin is an curcuminoid compound of turmeric. Curcumin has been demonstrated to have anti-oxidant and anti-inflammatory properties. Bleomycin (BLM) is an anti-cancer drug which induced scleroderma and pulmonary fibrosis in human and animals. Here we investigate biological effects of curcumin on bleomycin-induced scleroderma and pulmonary fibrosis in mice through pathomorphological assesment. In this study, each group consisted of 4-6 mice ddy strain, that groups namely (i) control, mice were subcutaneously (SC) injected with 100 μl sterilized aquadest in dorsal skin, (ii) bleomisin (BLM) group, injected with 100 μl of 1 mg/ml BLM SC in dorsal skin, (iii) curcumin (CMN) group, mice were intraperitoneally (IP) injected with 100 mg/kg body weight (BW) curcumin dissolved in 0.5% carboxymethylcellulose (CMC) and injected with 100 μl sterilized aquadest SC, (iv) BLM+CMN group, injected with 100 μl of BLM 1 mg/ml SC and injected with 100 mg/kg BW CMN in 0.5% CMC IP. Injections of PBS, BLM, CMN were performed daily for four weeks period. The mice were euthanized with overdosed ketamine, and afterwards the dorsal skin and lung samples were collected and fixed in 10% buffered neutral formalin (BNF). Histopathological evaluation was performed with hematoxylin-eosin (HE) and Masson’s trichrome (MT) stains. The results showed that BLM treatment significantly decreased hair follicles number and size. Additionally, in BLM-treated group there was significant increase in skin fibrosis area and inflammatory cells (macrophage and lymphocyte) number. In the lung, repeated BLM treatment initiated higher fibrosis area and alveolar wall area fraction as compared with control. In the other hand, CMN treatment results significantly increased hair follicles number and size, significantly reduced skin fibrosis area, lower inflammatory cells number, and significantly decreased fibrosis area, reduced alveolar wall area fraction in BLM-treated mice. Conclusively, our study showed that CMN treatment may inhibit skin and lung fibrogenesis in BLM-induced scleroderma and pulmonary fibrosis.