TNF-a Production Inhibitory Activity, Phenolic, Flavonoid and Tannin Contents of Selected Indonesian Medicinal Plants
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Date
2012Author
I. Batubara
S. Kotsuka
K. Yamauchi
H. Kuspradini
T. Mitsunaga
L.K. Darusman
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In this study 28 Indonesian medicinal plants species has been screened as potential Tumor Necrosis Factor (TNF)-a inhibitor. Plant materials were extracted with methanol. The activity test was performed on human monocyte-derived THP-1 (ATCC No. TIB 202) which was stimulated by Lipopolysaccharide (LPS). The TNF-a production and cell viability were determined by commercial ELISA kit and WST-1, respectively. All extracts were determined for their phenolic, flavonoid and tannin contents by spectrophotometry and chromatography methods. From 40 plant materials, 5 plant materials had cell viability lower than 70% at 10 ppm concentration and about 8 plant materials had good cell viability higher than 90% at 10 ppm concentration. Among 8 plant materials extract which had good cell viability, only Avicennia sp. stem and Goniothalamus macrophyllus leaf methanol extracts had high TNF-a inhibitory activity. The total phenolic, flavonoid and tannin contents were varied depending on the sample. No correlation was found between TNF-a inhibitory activity with the total phenolic content, flavonoid content and tannin content. In conclusion, among Indonesian medicinal plants, the highest potential as TNF-a inhibitors were Avicennia sp. stem and G. macrophyllus leaves.