Bacterial communities associated with white shrimp (Litopenaeus vannamei) larvae at early developmental stages

Abstract

Bacterial communities associated with white shrimp (Litopenaeus vannameij larvae at early developmental stages. Biodiversitas 11 (2): 65-68.Terminal Restriction Fragment Length Polymorphism (T-RFLP) was used to monitor the dynamics of the bacterial communities associated with early developmental stages of white shrimp (Litopenaeus vanname1) larvae. Samples for analysis were egg, hatching naupli~ 24 hours old nauplii, and 48 hours old nauplii which were collected from one cycle of production at commercial hatchery. TRFLP results indicated that the bacterial community associated with early stages of shrimp development might be transferred vertically from broodstock via egg. There was no significant difference between bacterial communities investigated, except the bacterial community of 48 hours old nauplii. Diversity analyses showed that the bacterial community of egg had the highest diversity and evenness, meanwhile the bacterial community of 48 hours old nauplii had the lowest diversity. Nine phylotypes were found at all stages with high abundance. Those TRFs were identified as r- proteobacteria, a-proteobacteria, and bacteroidetes group.