Metode Pengusangan Cepat dengan Larutan Etanol untuk Pengujian Vigor Daya Simpan Benih Caisin (Brassica rapa L. cv. grup Caisin)

Abstract

The objective of this study was to obtain effective duration in accelerated aging method with soaking the seed in 20% liquid ethanol that can estimate vigor related to storability caisin seed during 3 and 6 months storage. There were five caisin seed lot used in this study. The seed were taken from five commercial seed with different initial vigor. This research was conducted in two separate experiments. The first experiment was conducted to study caisin seed that stored in aluminium foil pouches during 3 and 6 months period at storage room with temperature 23.3−29.9 °C and 61−85% relative humidity. The second experiment was conducted to study the effect from different duration soaking of the seed that has been moistened for 12 hours in liquid ethanol 20% for 30, 60, 90, 120, 150, and 180 minutes. Both of experiments were arranged in completely nested design. Germination of seed after soaking in 20% liquid ethanol for 90 minutes positively correlated with germination of seed after storage for 3 and 6 months with a coefficient of corellation 0.87 and 0.88. Both the coefficient of corellation close to 1 which shows that the germination of seed after soaking in 20% liquid ethanol can estimate vigor related to storability seed. Vigor related to storability caisin seed after 3 month can be predicted by the equation y = 56.04 + 0.36x with the coefficient of determination 77.00%. Vigor related to storability caisin seed after 6 month can be predicted by the equation y = 62.72 + 0.38x with the coefficient of determination 74.90%. Variable y indicates germination of caisin seed after storage while the variable x indicates germination of caisin seed after soaking in 20% liquid ethanol for 90 minutes.