Pengujian Bacillus thuringiensis galur SAHA 12.08 sebagai pengendali pertumbuhan cendawan penyebab bercak daun kelapa sawit

Abstract

Oil palm (Elaeis guineensis Jacq.) is one of major agriculture commodities in Indonesia. The disease caused by pathogenic fungi such as Curvularia affinis and Colletotrichum gloeosporoides could inhibit the growth of oil palm seedling plants. Cell wall, mycelia cells, stalks, and conidium of fungi that composed by chitin, can be degraded by chitinolytic bacteria through its ability to produce the chitinase enzyme. This research was aimed to study the ability of Bacillus thuringiensis strain SAHA 12.08 in inhibiting C. affinis and C. gloeosporoides that attacked oil palm leaf through in vitro experiment. Bacillus thuringiensis strain SAHA 12.08 showed inhibitor activity to pathogenic fungi using 24 hour and 12 hour bacteria culture. Result of inhibition test using Detached-Leaf Assays method showed that capability of Bacillus thuringiensis as biocontrol activity against C. affinis was 85% and 90%, and against C. gloeosporoides was 96% and 59%. Inhibition of the strain against C. affinis and C. gloeosporoides using in vitro test were 27,8% and 28,2 % respectively. Bacillus thuringiensis strain SAHA 12.08 had antagonistic activity at in vitro test using leaf of oil palm and showed biocontrol activity against C. affinis and C. gloeosporoides