Pembuatan Prebiotik Manooligosakarida (MOS) serta pengujiaanya Terhadap Pertumbuhan Salmonella sp. dan Pediococcus pentosaceus

Abstract

Prebiotic was recomended as a substitute for antibiotics in the feed ingredients. One of prebiotics is mannooligosaccharide (MOS). MOS is the result of bacterial degradation from mannose containing substrates. The aim of this research is to analyze the ability of the W6 to produce mannooligosaccharide prebiotics from palm kernel cake (PKC) and test it against the growth of lactic acid and pathogenic bacteria. The analyses were performed with the clearing zone test, enzyme activity, substrate concentration election of palm kernel cake (PKC), and the growth of lactic acid and pathogenic bacteria tests. Isolate W6 had mannanolytic index of 6.5. The results of the clearing zone showed a transparant red color. Isolate W6 had several peaks of enzyme activity, at 24th, 30th , 48th , 78th , and 96th hours of culture incubation. Highest mannanase activity of isolate W6 at pH 5.5 and 30° C with incubation time of 96 hours was 0.0265 U/mL. The isolate was able to produce the best MOS with PKC concentration was 10%. The prebiotic MOS increase the growth of Pediococcus pentosaceus and was able to inhibit growth of Salmonella sp. It is concluded that W6 has the degree of polymerization at 3.3-8.6 in production of MOS as prebiotic.