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      • Research Journal :: Jurnal Ilmu Pertanian Indonesia
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      Spesies nematoda puru akar (meloidogyne spp.) Yang berasosiasi dengan penyakit umbi bercabang pada wortel: penyakit baru di indonesia

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      Date
      2012
      Author
      Suastika, Gede
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      Abstract
      Diseases and pests is one of the main obstacles in the cultivation of carrots in Indonesia. One of the emerging diseases that cause significant losses is branched (forked) tuber caused by root knot nematodes (RKN), Meloidogyne spp. Research aimed to identify the species of RKN was carried out by taking samples of diseased plants and soil from the carrot plantation in four vegetable production centers on the island of Java, namely: (1) District Pacet, Cianjur, West Java, (2) Dieng Plateau, Central Java, (3) Kopeng, Central Java and (4) District Bumiaji, Kota Batu, East Java. Three carrot plantations with different altitude / elevation were sampled for each area involved. NPA species identification was done by observation of perineal pattern (fingerprint-like pattern) of females and PCR of nematodes r-DNA ITS. Four Meloidogyne species, namely M. arenaria, M. hapla, M. incognita, and M. jananica, were identified from West Java, Central Java and East Java samples, whereas M. fallax found only in West Java.
       
      Penyakit dan hama merupakan salah satu kendala utama dalam bertanam wortel di Indonesia. Penyakit baru yang menimbulkan kerugian besar adalah penyakit umbi bercabang yang disebabkan nematoda puru akar (NPA) Meloidogyne spp. Penelitian untuk mengidentifiksi species NPA telah dilakukan dengan mengambil sampel tanaman sakit dan tanah dari pertanaman wortel di empat sentra produksi sayuran di Pulau Jawa, yaitu (1) Kecamatan Pacet, Cianjur, Jawa Barat, (2) dataran tinggi Dieng, Jawa Tengah, (3) Kopeng, Jawa Tengah, dan (4) Kecamatan Bumiaji, Kota Batu, Jawa Timur. Tiga lahan pertanaman wortel dengan ketinggian / elevasi yang berbeda diambil sebagai contoh untuk setiap wilayah pengamatan. Identifikasi spesies NPA dilakukan dengan pengamatan pola perineal (sidik pantat) betina dan PCR ITS r-DNA nematoda. Empat spesies Meloidogyne, yaitu M. arenaria, M. hapla, M. incognita, dan M. jananica, berhasil diidentifikasi dari sampel asal Jawa Barat, Jawa Tengah dan Jawa Timur, sedangkan M. fallax hanya ditemukan di Jawa Barat.
       
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      http://repository.ipb.ac.id/handle/123456789/71543
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      • Research Journal :: Jurnal Ilmu Pertanian Indonesia [151]

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      Copyright © 2020 Library of IPB University
      All rights reserved
      Contact Us | Send Feedback
      Indonesia DSpace Group 
      IPB University Scientific Repository
      UIN Syarif Hidayatullah Institutional Repository
      Universitas Jember Digital Repository