Peran Infusa Buah Adas (Foeniculum vulgare Mill.) terhadap Kinerja Reproduksi Tikus Betina Umur 1 Tahun

Abstract

Fennels are plant that contains phytoestrogen with effect like estrogen endogenous. This study aimed to know about administration of sweet fennels to observe reproductive performance such as an estrous cycle, weight of ovaries and uterine, and uterine vascularitation from 1 year old white female rats. This research used 25 female rats that were divided in 5 groups. KN is negative control given 1 ml/100 gBW destilated water. KP is positive control given etinil estradiol 0.045 mg/200 gBW. Treatment doses D1, D2, and D3 given infusion of 36.5 mg, 73 mg and 146 mg every 100 gBW fennel respectively. Treatment of destilated water, etinil estradiol and fennels infusion were done for 17 days with the oral route, at the same time with vaginal swab. Vaginal swab were taken 2 times a day with a period of 12 hours, to study the length of estroes cycle and its phase. After given treatment, the rats were necropsied to observe uterine vascularitation and weight of the ovaries and uterine. The changes of vaginal epithelium were examined to determine estrous cycle phase using a microscope. It was concluded that fennel fruit infussion at dose D1 can increased length of proestrus (P<0.05) and it can increased length of estrous phase (P>0.05). There were increasement of the ovaries and uterine weight from rats group KP, D1, and D2 (P>0.05), and D3 (P<0.05), however all groups did not show increasement of uterine vascularitation.