Analisis Selen Dalam Susu Bubuk Menggunakan Spektrometri Emisi Atom-Plasma Gandeng Induktif dan Spektrometri Serapan Atom Nyala

Abstract

Selenium is an essential micromineral which can be toxic in high doses. This research compared 2 methods of selenium analysis in milk powder, namely by inductively coupled plasma-atomic emission spectrometry (ICP-AES) and flame atomic absorption spectrometry (FAAS), based on the linearity, limit of detection and quantification, precision, accuracy, and robustness, and measurement of 4 milk powder samples with different brand. Both ICP-AES and FAAS methods had good linearity with coefficient of determination higher than 0.99. Limit of detection and quantification for ICP-AES were 0.444 and 1.480 μg/L, not as good as FAAS which were 0.105 and 0.350 μg/L, respectively. In the other hand, the ICP-AES method was more precise and accurate than FAAS, indicated by lower relative standard deviation value and by recovery value ranging between 80 and 110%. Robustness test showed that destruction time effected the selenium concentration measured by ICP-AES, but had no effect on FAAS measurement. Four milk powder samples showed 4‒84% difference obtained between measurement ICP-AES and FAAS. From these results, the more precise and accurate ICP-AES methods was more recommended for selenium content measurement in milk powder.