Toksisitas Ekstrak Senyawa Bioaktif dari Bakteri yang Berasosiasi dengan Spons dan Analisis DNA yang Berperan dalam Biosintesisnya

Abstract

Sponges are organisms that produced many bioactive compounds and live symbiotically with many microorganisms. Symbiont microorganisms act as producers of bioactive compounds for sponge self-defense. This study aims to test the toxicity of the ethyl acetate extract sponge-associated bacteria against Artemia salina larvae and diversity analysis of DNA that play a role in the synthesis of bioactive compounds. BSLT was used as toxicity assay method and obtained LC50 values ranged from 117.1 μg/mL to 620 μg/mL. Fractionation of the ethyl acetate extract for isolates SAB E-35, SAB E-38 and SAB E-40 using preparative thin layer chromatography technique (PTLC) yielded fraction with the best LC50 value 116.7 μg/mL. Fraction 38-E of isolate SAB E-38 showed increase in LC50 value from its etyl acetat extract 138.6 μg/mL to 116.7 μg/mL. Isolates SAB E-35, SAB E-38, and SAB E-40 has gene that encoded the KS domain of PKS and A domain of NRPS with homology percentage from 47% to 100%. SAB E-40 has low homology and expected as potential producer of new bioactive compounds