Pengembangan Formulasi Biopestisida Berbahan Aktif Bacillus subtilis AB89 dan Staphylococcus epidermidis BC4 untuk Mengendalikan Penyakit Layu Bakteri pada Tomat

Abstract

Ralstonia solancearum is a pathogen of bacterial wilt disease on tomato. One of the alternatives for disease control is the application of biocontrol agents. Biocontrol agents which have been able to control the disease are Bacillus subtilis AB89 (PGPR) and Staphylococcus epidermidis BC4 (endophyte). This research was conducted to evaluate the viability of those bacteria in formulation and the effectiveness of the formulation to control the bacterial wilt disease of tomato. B. subtilis AB89 (BS) and S. epidermidis BC4 (BC) ware formulated singly in liquid (BS and BC) and powder (TBS and TBC) formulation contained talc powder and/or xanthan gum. The liquid formulation of B. subtilis AB89 (BS) and S. epidermidis BC4 (BC) effectively suppressed the incidence of the disease, with control effectiveness was up to 55.55% and 54.94%, respectively. The liquid formulation BC, BS and the powder formulation TBS were able to promote the plant growth. Populations of S. epidermidis BC4 was stable within powder and liquid formulations during 8 weeks of storing. Population of B. subtilis AB89 in powder and liquid formulations declined on 1 week after storage, but stable until 8 weeks after storage.