Aplikasi Selulosa-g-Poliakrilamida sebagai Fase Diam Kromatografi Kolom dan Sintesisnya Melalui Reaksi Kopolimerisasi Cangkok dan Taut-Silang

Abstract

The graft and crosslink copolymerization of acrylamide to cellulose isolate have been carried out using N,N’-methylene-bis-acrylamide (MBA) as crosslinker and ammonium peroxydisulfate as initiator. The amount of MBA added were 1.0, 1.5, and 2.0 g. Grafting ratio and efficiency of cellulose-g-polyacrylamide using MBA 2.0 g were the highest, while water absorption capacity was the lowest. Infrared spectrum showed the presence of amide and hydroxyl as well as ether functional groups. Pore size distribution of the product was classified as mesoporous (3.6 nm in diameter). Scanning electron micrograph showed a more tight surface morphology on cellulose-g-polyacrylamide with addition of 2.0 g MBA. Performances of the product were evaluated by high performance liquid chromatography using Curcuma xanthorrhiza extract. The selectivity and resolution value of xanthorrhizol separation in the stationary phase synthesized from 2.0 g MBA addition were 1.276 and 1.870, respectively. Nuclear magnetic resonance spectrum and specific rotation value of the isolated xanthorrhizol proved that the stationary phase did not change the structure and the three-dimensional configuration of the xanthorrhizol, so that it can be proposed as a highly useful value in commercial development.