Seleksi bakteri endofit untuk pengendalian penyakit rebah kecambah (Pythium sp.) pada tanaman mentimun

Abstract

Damping-off is one of the major diseases that often attack almost any plants in the nursery phase. Cucumber is one of the main hosts for damping-off disease caused by Pythium sp. The use of biocontrol agents such as endophytic bacteria is one an alternative for controlling plant diseases. The objective of this study was to isolate an endophytic bacteria from betel and paddy and to select their potency in controlling damping-off disease on cucumber. The isolation of endophytic bacteria was carried out through surface sterilization method with 70% alcohol, 4% NaOCl and sterile distilled water. A total of 50 isolates of endophytic bacteria were isolated from the betel and two isolates from the paddy. Based on antibiosis test result, growth and suppression of endophytic bacteria against damping-off, four isolates AS2, BS14, Ci6, and Ci10 were known to have ability in suppressing the disease severity of damping-off up to 87.6% and able to increase the plant growth of cucumber in a greenhouse.